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目的探讨阿司匹林对小鼠胚胎腭裂发生的作用,确立阿司匹林诱导小鼠腭裂模型的最佳剂量,为深入研究腭裂提供动物实验模型。方法分别将确定孕期的BALB/C雌鼠随机分为5组,即对照组和实验Ⅰ~Ⅳ组。胚胎第10~12天(embryo 10~embryo 12,E10~E12)时,每日定时给予对照组孕鼠灌胃生理盐水,实验Ⅰ、Ⅱ、Ⅲ、Ⅳ组孕鼠分别灌胃阿司匹林180、210、240、270mg/kg。于E18时处死孕鼠,称量并计算孕鼠和胚胎的体质量,记录胚胎腭裂数及发育情况。结果对照组腭裂发生率明显低于实验Ⅲ、Ⅳ组,差异有统计学意义(χ2=15.057,P<0.01;χ2=17.551,P<0.01)。实验Ⅲ、Ⅳ组比较,实验Ⅲ组腭裂发生率高于实验Ⅳ组,差异有统计学意义(χ2=4.742,P<0.01);各组间胚胎体质量的差别无统计学意义(P>0.05)。结论阿司匹林可致小鼠胚胎腭裂发生,240mg/kg阿司匹林是建立BALB/C胎鼠腭裂模型的最佳剂量。
Objective To investigate the effect of aspirin on cleft palate in mice and to establish the best dose of aspirin-induced cleft palate in mice, and to provide an experimental model for the further study of cleft palate. Methods The BALB / C female mice during pregnancy were randomly divided into 5 groups: control group and experimental group Ⅰ ~ Ⅳ. Embryos 10 to 12 days (embryo 10 ~ embryo 12, E10 ~ E12), the control group were given daily time-lapse physiological saline, experimental Ⅰ, Ⅱ, Ⅲ, Ⅳ pregnant rats were administered aspirin 180,210 , 240,270 mg / kg. The pregnant rats were sacrificed at E18, the body weight of pregnant rats and embryos was weighed and counted, and the cleft palate number and development of embryos were recorded. Results The incidence of cleft palate in the control group was significantly lower than that in the experimental group Ⅲ and Ⅳ (χ2 = 15.057, P <0.01; χ2 = 17.551, P <0.01). The incidence of cleft palate in experimental group Ⅲ was higher than that in experimental group Ⅳ (χ2 = 4.742, P <0.01). There was no significant difference in the embryo mass between the three groups (P> 0.05 ). Conclusion Aspirin can induce the cleft palate in mice. 240 mg / kg aspirin is the best dose to establish BALB / C fetal rat palate model.