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以槲皮素作为模板分子,邻氨基苯酚作为功能单体,在金电极表面通过电聚合法,制备了具有选择性识别槲皮素的分子印迹传感器。采用循环伏安法、差分脉冲伏安法研究了印迹膜的性能、结构和分子印迹效应。对功能单体与模板分子的配比、洗脱时间和印迹时间等实验参数进行了优化,并与其结构相似的化合物芦丁的选择性响应进行了比较,发现该传感器对槲皮素分子具有良好的选择性。槲皮素浓度在6.0×10-6~1.0×10-4mol/L范围内与峰电流呈线性关系,线性方程为:I(μA)=27.79+9.48lgc(mol/L)(R=0.9939),检出限为2.0×10-6mol/L。用此传感器测定黑茶中槲皮素含量的结果较为满意。
Using quercetin as template molecule and o-aminophenol as functional monomer, a molecular imprinting sensor with selective recognition of quercetin was prepared by electropolymerization on the surface of gold electrode. Cyclic voltammetry and differential pulse voltammetry were used to investigate the performance, structure and molecular imprinting effect of imprinted films. The experimental parameters such as the ratio of the functional monomer to the template molecule, the elution time and the imprinting time were optimized and compared with the selective response of the structurally similar compound rutin, and it was found that the sensor had good quercetin molecule The selectivity. Quercetin concentration in the range of 6.0 × 10-6 ~ 1.0 × 10-4mol / L was linear with the peak current, the linear equation was: I (μA) = 27.79 + 9.48lgc (mol / L) (R = 0.9939) , The detection limit was 2.0 × 10-6mol / L. The results of the determination of quercetin in black tea by this sensor are satisfactory.