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通过离体选择抗赖氨酸加苏氨酸玉米突变体,不仅获得了积累游离苏氨酸和赖氨酸等氨基酸的突变类型,也选择到了种子蛋白组份发生改变的高蛋氨酸突变体和高赖氨酸体细胞无性系变异体。高蛋氨酸种子中总蛋氨酸的含量比对照增高22.6%,这是由于醇溶蛋白Zein-2部分及其蛋氨酸含量增高所致。高赖氨酸变异体种子游离必需氨基酸增高显著(2-10倍),总赖氨酸比对照亲本增高28.1%,含量约占种子干重的0.40%,种子蛋白清蛋白、球蛋白和谷蛋白组份含量增高而醇溶蛋白减少。高赖氨酸和高蛋氨酸特性遗传稳定,植株育性正常。
By in vitro selection of mutants resistant to lysine and threonine, not only the types of mutations that accumulate amino acids such as free threonine and lysine were obtained, but also the high methionine mutants that were altered in seed protein composition and the high Lysine somatic clones variants. The content of total methionine in the high-methionine seeds increased by 22.6% over the control, due to the increase of the zein-2 part of gliadin and its methionine content. Highly lysine variant seeds significantly increased the free essential amino acids (2-10 times), total lysine 28.1% higher than the control parent, the content of about 0.40% of the seed dry weight, seed albumin, the ball Increased protein and gluten components and reduced gliadin. High lysine and high methionine genetic stability, plant fertility normal.