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毛叶杜鹃叶片的不定芽分化是最节约的取材方法,至今国内外尚未报道过。在秋季或春季,从生长中的毛叶杜鹃的植株上剪下叶片,用皂水洗涤,再用自来水冲洗数次。然后将叶片浸没在7％漂白精片清液中,灭菌半小时。在无菌条件下,用无菌水将上述叶片冼3次,然后用镊子将整张叶片放在1/1Ms+Z 2mg/1(Z:玉米素)培养基上,进行静置培养。
Mucous rhododendron leaves adventitious bud differentiation is the most economical method of drawing, has not been reported at home and abroad. In the fall or spring, cut the leaves from growing plants of the cuckoo, wash with soapy water and rinse with tap water several times. The leaves were then immersed in 7% bleached serum and sterilized for half an hour. Under aseptic conditions, the leaves were washed three times with sterile water and the whole leaf was placed on a 1 / 1Ms + Z 2mg / 1 (Z: zeatin) medium using forceps for static culture.