论文部分内容阅读
目的:观察新风胶囊(XFC)对佐剂关节炎(AA)大鼠肺功能、调节T细胞(Treg)及肺组织Foxp3、TGF-β1、Smad3、Smad7的影响。方法:采用弗氏完全佐剂复制AA大鼠模型,致炎后第19天开始给药,将大鼠随机均分为正常对照(NC)组、模型对照(MC)组、甲氨喋呤(MTX)组、雷公藤多苷片(TPT)组和XFC组,给药30 d后,观察各组大鼠足趾肿胀度(E)、关节炎指数(AI)、肺功能、Treg、肺组织病理形态学及肺组织Foxp3、TGF-β1、Smad3、Smad7变化。结果:与NC组相比,MC组大鼠E、AI、肺泡炎积分、TGF-β1及Smad3蛋白表达明显升高(P<0.05或P<0.01);25%肺活量的最大呼气流量(FEF25)、50%肺活量的最大呼气流量(FEF50)、75%肺活量的最大呼气流量(FEF75)、最大呼气中期流量(MMF)、用力最大呼气流量(PEF)、CD4+CD25+Treg及Foxp3、Smad7蛋白表达显著降低(P<0.05或P<0.01)。与MC组相比,XFC组大鼠E、AI、TGF-β1及Smad3表达降低,FEF50、FEF75、MMF、PEF、Treg及Foxp3、Smad7表达升高(P<0.05或P<0.01)。与XFC组相比,MTX组、TPT组大鼠体质量、FEF25、FEF50、FEF75、MMF、Treg降低(P<0.05或P<0.01)。结论:AA大鼠存在关节炎症症状和肺功能降低,XFC能明显抑制AA大鼠足跖肿胀度,降低关节炎症反应,改善肺功能,机制可能是通过上调Foxp3、Treg,下调TGF-β1表达,调节TGF-β1/Smads信号通路传导,改善关节症状和肺功能。
Objective: To observe the effect of Xinfeng Capsule (XFC) on the lung function, Treg and the expression of Foxp3, TGF-β1, Smad3 and Smad7 in adjuvant arthritis (AA) rats. Methods: AA model was induced by complete Freund ’s adjuvant (AR). After 19 days of inflammation, the rats were randomly divided into normal control group (NC), model group (MC), methotrexate MTX group, tripterygium glycosides tablet (TPT) group and XFC group. After administration for 30 days, the degree of swelling of the toes (E), arthritis index (AI), lung function, Treg, lung tissue Pathological changes and lung tissue Foxp3, TGF-β1, Smad3, Smad7 changes. Results: Compared with NC group, the expression of E, AI, alveolitis, TGF-β1 and Smad3 in MC group were significantly increased (P <0.05 or P <0.01); the maximal expiratory flow rate in 25% , Maximum expiratory flow (FEF50) at 50% vital capacity, maximum expiratory flow at 75% vital capacity (FEF75), maximum expiratory flow (MMF), maximum forced expiratory flow (PEF), CD4 + CD25 + Treg and Foxp3, Smad7 protein expression was significantly lower (P <0.05 or P <0.01). Compared with the MC group, the expression of E, AI, TGF-β1 and Smad3 in the XFC group decreased and the expression of FEF50, FEF75, MMF, PEF, Treg, Foxp3 and Smad7 increased (P <0.05 or P <0.01). Compared with XFC group, the body weight, FEF25, FEF50, FEF75, MMF and Treg in MTX group and TPT group decreased (P <0.05 or P <0.01). Conclusions: The arthritis symptom and lung function of AA rats are decreased. XFC can significantly reduce the degree of plantar swelling, decrease the inflammatory reaction and improve the lung function in AA rats. The mechanism may be through up-regulating Foxp3 and Treg, down-regulating the expression of TGF-β1, Regulate the TGF-β1 / Smads signaling pathway to improve joint symptoms and lung function.