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目的探讨奥硝唑注射液对体外培养的齿龈内阿米巴活力的影响。方法于2010年6—8月在青岛大学医学院附属医院口腔科,使用蛋白胨-酵母-葡萄糖(PYG)培养液体外培养齿龈内阿米巴,随机从来源于不同患者的6份阿米巴悬液中各取6mL,在实验前以及加入奥硝唑注射液1、2、5、10、30min从每份中取1mL,用台盼蓝染色确定各份阿米巴的活力;分别在每个检测时间点选择活力最强的悬液,离心去上清后加入PYG培养液培养5d,并观察其增殖情况及形态变化。结果奥硝唑分别作用1、2、5min后,阿米巴活力与实验前比较,差异无统计学意义(P>0.05);奥硝唑分别作用10min和30min后,阿米巴活力与实验前比较,差异有统计学意义(P<0.05)。离心并培养1个周期后观察显示,奥硝唑作用1、2min的阿米巴增殖状况良好,作用5、10、30min的阿米巴增殖力减弱甚至消失。结论奥硝唑作用10min以上杀灭齿龈内阿米巴的效果较好,口腔内局部使用该药物可以成为治疗齿龈内阿米巴感染的有效手段。
Objective To investigate the effect of ornidazole injection on the activity of Amoeba gingivalis cultured in vitro. Methods From June to August in 2010, we conducted an in vitro study on the clinical application of peptone-yeast-dextrose (PYG) cultured liquid in the Department of Stomatology, Affiliated Hospital of Medical College of Qingdao University, and randomly selected six amoeba suspensions from different patients Each taking 6mL of liquid before taking the test and adding ornidazole injection 1, 2, 5, 10, 30min from each taking 1mL, trypan blue staining to determine the activity of each amoeba; respectively At the time of detection, the strongest suspension was selected, then the supernatant was centrifuged and then cultured in PYG for 5 days. The proliferation and morphological changes were also observed. Results After ornidazole were applied for 1, 2 and 5 min respectively, the viability of amoebic was not significantly different from that before the experiment (P> 0.05). After 10 and 30 min of ornidazole treatment, The difference was statistically significant (P <0.05). After centrifugation and culture for 1 cycle, the results showed that the proliferation of amebic amiodarone was good at 1 and 2 min, and the proliferation of amoeba at 5, 10 and 30 min was weakened or even disappeared. Conclusion The effect of ornidazole on killing alveolar gingivalitis is better than 10 minutes. Local use of the drug in oral cavity can be an effective method for the treatment of infections of the gingivalitis.