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目的探讨来氟米特(LEF)对滑膜病变的治疗作用。方法 40只雌性SD大鼠用完全弗氏佐剂乳化的鸡Ⅱ型胶原构建胶原诱导性关节炎(CIA)大鼠关节模型后均分为模型对照(B)组和LEF处理组(A组,LEF 1mg/d灌胃,连续3周);另取10只SD大鼠作为正常对照(C)组。进行关节炎指数(AI)评分,ELISA法检测关节浸液IL-17和细胞核因子κB受体活化因子配基(RANKL)浓度,计算膝关节滑膜病理积分,免疫组化法半定量检测滑膜IL-17和RANKL蛋白表达。结果 A组于给药2周后的AI值低于B组(6.60±1.65vs.9.90±2.38)(P<0.01)。与C组比较,A、B关节浸液IL-17和RANKL浓度、滑膜病理积分、滑膜IL-17和RANKL蛋白表达均增加(P<0.05、P<0.01),但A组上述指标均低于B组(P<0.01)。结论 LEF可能通过抑制IL-17和RANKL在CIA大鼠关节滑膜的表达延缓关节滑膜病变的进展。
Objective To investigate the therapeutic effect of leflunomide (LEF) on synovial lesions. Methods Forty female Sprague - Dawley rats were divided into two groups: model control group (B) and LEF group (group A, LEF 1mg / d gavage for 3 weeks); another 10 SD rats as a normal control (C) group. The arthritis index (AI) was scored. The concentrations of IL-17 and RANKL in the joint infiltration were detected by ELISA. The synovial pathological scores were calculated. The synovial membrane was detected by immunohistochemistry IL-17 and RANKL protein expression. Results The AI of group A after 2 weeks of administration was lower than that of group B (6.60 ± 1.65 vs.9.90 ± 2.38) (P <0.01). Compared with group C, IL-17 and RANKL concentration, synovial pathological score, synovium IL-17 and RANKL protein expression in group A and B joint infiltrates were increased (P <0.05, P <0.01) Lower than B group (P <0.01). Conclusion LEF may delay the progression of synovial lesions by inhibiting the expression of IL-17 and RANKL in the synovial membrane of CIA rats.