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目的通过研究益肾健脾活血法对ＢＥＬ－７４０２肝癌细胞药理学作用，探讨中药血清药理学抗肿瘤体外实验途径。方法取家兔随机分为大、中、小中药剂量组和空白对照组，每组６只，中药组分别予益肾健脾活血中药制剂６、３、１ｇ／ｋｇ，空白对照组予生理盐水灌胃，定时采集血清用于体外实验。结果显示含中药兔血清可明显抑制ＢＥＬ－７４０２细胞增殖，最高抑制率达５７．５％，并且相同时间点高剂量组抑制率高于低剂量组、相同剂量组长时间点高于短时间点。结论以益肾健脾活血法制定的中药制剂确含抑制ＢＥＬ－７４０２细胞增殖有效成分，并且此成分口服有效；同时表明采用血清药理学方法进行抗肿瘤中药体外试验，具有一定的科学性和可行性
Objective To study the pharmacological effects of Yishen Jianpi Huoxue method on BEL-7402 hepatocellular carcinoma cells, and explore the anti-tumor in vitro experimental methods of serum pharmacology of traditional Chinese medicine. Methods Rabbits were randomly divided into large, medium, and small doses of traditional Chinese medicine and blank control group. Each group consisted of 6 rats. The traditional Chinese medicine group received Yishen Jianpi Huoxue Chinese medicine preparations of 6, 3, 1g/kg, and the blank control group received normal saline. Gavage, regular collection of serum for in vitro experiments. The results showed that rabbit serum containing traditional Chinese medicine can significantly inhibit the proliferation of BEL-7402 cells, the highest inhibition rate was 57.5%, and the inhibition rate was higher in the high-dose group at the same time than in the low-dose group, the long-term point higher than the short-time point in the same dose group. . Conclusion The traditional Chinese medicine preparation formulated with yin and spleen promoting blood circulation method does contain effective ingredients for inhibiting the proliferation of BEL-7402 cells, and this ingredient is orally effective. It also shows that the use of serum pharmacology for in vitro experiments of anti-tumor herbs is scientific and feasible. Sex