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目的 探索日本血吸虫基因组内是否存在DNA重复序列。方法 分别根据曼氏血吸虫基因组特异的DNA重复序列单位Sm1- 7和埃及血吸虫基因组特异的DNA重复序列单位TheDraIRepeat设计并合成了寡核苷酸引物 ,然后以日本血吸虫基因组DNA为模板 ,用自备的多达 12种的反应缓冲液进行多聚酶链式反应 (PCR)优化扩增。结果 分别成功地从日本血吸虫基因组中扩增出与曼氏血吸虫基因组“特异的”DNA重复序列单位Sm1- 7和埃及血吸虫基因组“特异的”DNA重复序列单位TheDraIRepeat同源的DNA重复序列。PCR反应提示该两种重复序列在日本血吸虫基因组内的拷贝数均较低。同时 ,日本血吸虫与曼氏血吸虫表现出较好的同源性。结论 日本血吸虫基因组内可能存在着长度为 12 1bp的DNA重复序列 ,进一步的确认尚有赖于DNA序列测定
Objective To explore the existence of DNA repeats in the genome of Schistosoma japonicum. Methods Based on the genome-specific DNA repeat unit Sm1-7 of Schistosoma mansoni and the genome-specific DNA repeat unit of Theraphis sp., The oligonucleotide primers were designed and synthesized. Then, Up to 12 reaction buffers are optimized for polymerase chain reaction (PCR) amplification. Results Sequences of DNA repeats homologous to the “specific” DNA repeat unit Sm1-7 of the Schistosoma mansoni genome and the “specific” DNA repeat unit TheDraIRepeat of the genome of S. schistosoma were successfully amplified from the genome of Schistosoma japonicum. The PCR reaction indicated that the copy number of the two repeats in the genome of Schistosoma japonicum was low. At the same time, Schistosoma japonicum and Schistosoma mansoni showed better homology. Conclusion DNA sequence of 12 1bp may exist in the genome of Schistosoma japonicum, and the further confirmation depends on DNA sequencing