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利用改进后的Ｔｂ／ＤＰＡ荧光方法对在脉冲电场作用下人血影内Ｔｂ离子外渗的动力学过程进行了系统的研究。对不同场强和脉宽的电场处理的离子外渗量与时间的关系和电穿孔总面积随时间的变化进行了测量。结果表明，离子外渗的主要方式是由膜两侧浓度梯度导致的离子自由扩散。当脉冲宽度或强度两者之一给定的情况下，均存在临界场强和临界脉宽，在临界点以上电场作用下，膜上出现明显的电穿孔。在实验条件下，电穿孔的面积在２００－３００ｍｓ时达最大值，孔的面积和扩展速度与电场参数有关。临界点以下电场处理后，虽仍能测出离子的外渗，膜上可能未出现明显的电穿孔。
The Tb / DPA fluorescence method was used to systematically study the kinetics of Tb ion extravasation in human plasma under pulsed electric field. The relationship between the extrapolation amount of the electric fields with different field strength and pulse width and time and the change of the total area of electroporation with time were measured. The results show that the main way of extracavity is the free diffusion of ions caused by the concentration gradient on both sides of the membrane. When either pulse width or intensity is given, there is critical field strength and critical pulse width. Under the electric field above the critical point, obvious electroporation occurs on the membrane. Under the experimental conditions, the area of electroporation reaches the maximum at 200-300ms, and the area and expansion speed of the pores are related to the electric field parameters. After the critical point below the electric field treatment, although the extravasation of ions can still be measured, the membrane may not appear obvious electroporation.