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目的:筛选陆英具有保肝降酶作用的优选提取工艺,并探讨陆英中保肝降酶的活性部位。方法:采用了小鼠急性CCl4肝损伤模型,对陆英不同提取工艺所得的提取物进行保肝降酶药效学筛选,并把筛选出的最佳工艺提取物用不同溶剂萃取法和大孔吸附树脂分离法对活性部位进行了探索。结果:陆英75%乙醇提取物能非常显著地对抗CCl4导致小鼠肝损伤,效果优于其他提取工艺;陆英75%乙醇提取物经乙酸乙酯萃取部分能减少CCl4导致小鼠肝损伤,效果明显弱于陆英75%乙醇总提物,而石油醚、正丁醇和水萃取部分则无明显效果;陆英75%乙醇提取物经大孔吸附树脂纯化后30%、60%和95%乙醇洗脱液的高剂量和低剂量组均能对抗CCl4导致小鼠肝损伤,但30%乙醇洗脱液的作用最显著,并呈现剂量依赖性,且效果与陆英75%乙醇总提物相似。结论:75%乙醇为陆英的优选提取溶剂,陆英75%乙醇提取物若经不同极性溶剂萃取,活性部位为乙酸乙酯萃取部分;若经大孔树脂纯化,则活性有效部分主要集中在30%乙醇洗脱液部分。
OBJECTIVE: To screen the optimal extraction process of Lu Ying with hepatoprotective and enzymatic effects, and to explore the active site of Lu Yingzhong’s hepatoprotective enzyme. METHODS: A mouse model of acute CCl4 liver injury was used to evaluate the pharmacodynamics of hepatoprotective and hypoglycemic extracts obtained from Lu Ying’s different extraction processes. The optimal process extracts were selected using different solvent extraction methods and macropore. The active site was explored by the adsorption resin separation method. RESULTS: Lu Ying’s 75% ethanol extract significantly against liver damage induced by CCl4 was superior to other extraction processes. Lu Ying’s 75% ethanol extract reduced the CCl4-induced liver injury in mice by extracting ethyl acetate. The effect was significantly weaker than Lu Ying’s 75% ethanol extract, while petroleum ether, n-butanol, and water extracts had no significant effect; Lu Ying’s 75% ethanol extracts were purified by macroporous adsorptive resins at 30%, 60%, and 95%. Both the high and low dose ethanol eluates were able to fight CCl4-induced liver injury in mice, but the 30% ethanol eluate was the most significant and dose dependent, and the effect was consistent with Lu Ying’s 75% ethanol total extract. similar. Conclusion: 75% ethanol is the preferred extraction solvent for Lu Ying. Lu Ying’s 75% ethanol extract is extracted with different polar solvents. The active site is ethyl acetate extraction part; if purified by macroporous resin, the active part is mainly concentrated. In the 30% ethanol fraction.