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目的:研究莪术油对中波紫外线(ultraviolet B,UVB)所致豚鼠皮肤损伤的治疗作用及其机制。方法:UVB照射豚鼠皮肤,建立氧化损伤模型(照射30 d,累积28.38 J.cm-2)。实验观察皮肤红斑形成、粗糙程度等外观变化及HE常规染色后光镜下表皮厚度和真皮中成纤维细胞数量的变化。酶法检测皮肤匀浆上清液中过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量及总抗氧化能力(T-AOC)。结果:①光镜下可见UVB照射组和基质空白组照射侧表皮增厚,成纤维细胞数量减少,而未照射侧未见此病理变化;莪术油治疗组照射侧未见表皮增厚,而成纤维细胞增多;但未照射侧未见此病理变化。②与正常组比较,UVB照射组和基质空白组照射侧皮肤中MDA含量明显增多,CAT、GSH-Px和SOD活性及T-AOC降低;莪术油治疗组照射侧MDA减少,CAT、GSH-Px、SOD活性及T-AOC增加;各组未照射侧无明显改变。结论:莪术油具有对UVB皮肤氧化损伤的治疗作用,其作用机制可能与提高抗氧化酶含量及清除体内自由基有关。
Objective: To study the curative effect and mechanism of zedoary oil on skin lesions of guinea pigs induced by ultraviolet B (UVB). METHODS: UVB irradiation was performed on the skin of guinea pigs to establish an oxidative injury model (30 days irradiation, cumulative 28.38 J.cm-2). Changes in the appearance of skin erythema, roughness, etc., and changes in epidermis thickness under light microscopy and fibroblasts in the dermis were observed experimentally. Enzymatic detection of catalase (CAT), glutathione peroxidase (GSH-Px), superoxide dismutase (SOD) activity and malondialdehyde (MDA) content in the supernatant of the skin homogenate Total antioxidant capacity (T-AOC). RESULTS: 1 Under the light microscope, the epidermal thickness of the irradiated side of the UVB irradiation group and the matrix blank group was decreased, and the number of fibroblasts was decreased, but this pathological change was not seen on the non-irradiated side; no epidermal thickening was observed on the irradiation side of the zedoary oil treatment group. Fibroblasts increased; but no pathological changes were observed on the non-irradiated side. 2Compared with the normal group, the MDA content in the irradiated side of the UVB irradiation group and the matrix blank group increased significantly, the activity of CAT, GSH-Px and SOD, and the T-AOC decreased; the MDA in the irradiated side of the curcuma oil treatment group decreased, and the CAT and GSH-Px decreased. , SOD activity and T-AOC increased; no significant changes in the non-irradiated side of each group. Conclusion: Curcuma oil has therapeutic effects on UVB skin oxidative damage, and its mechanism may be related to the increase of antioxidant enzyme content and clearance of free radicals in vivo.