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用番茄‘Bonny Best’品种的子叶和下胚轴作为外植体,在MS附加不同水平的BA和激素的培养基中,愈伤组织形态发生的情况是不同的。本试验结果表明:在相同的植物生长调节剂水平下,番茄子叶外植体较下胚轴外植体更容易形成愈伤组织和生根;番茄子叶和下胚轴愈伤组织的形成要求不同的激素水平,较高的水平适合于子叶愈伤组织的生长,而下胚轴愈伤组织的生长要求较低的激素水平;较高浓度的BA诱导子叶和下胚轴外植体形成白色紧密的愈伤组织,而且茎的分化出现较早,较低水平的BA产生疏松的愈伤组织,分化效迟;在MS+BA22.2-44.4μM+IAA1-5μM的培养基上,番茄子叶和下胚轴切段可直接分化成茎;在无激素MS培养基上,下胚轴切段可直接成苗;疏松的愈伤组织是进行悬浮培养的良好材料,可利用它们进行原生质体培养和抗性克隆的筛选。
In cotyledons and hypocotyls of tomato ’Bonny Best’ as explants, morphological changes of callus were different in MS supplemented with different levels of BA and hormones. The results showed that the explants of tomato cotyledons were easier to form callus and root than the hypocotyl explants under the same level of plant growth regulator. The formation of tomato cotyledon and hypocotyl calluses required different Hormone levels, higher levels are suitable for cotyledon callus growth, whereas hypocotyl calluses require lower hormone levels; higher concentrations of BA induce cotyledon and hypocotyl explants to form white, compact The differentiation of callus and stem appeared earlier, the lower level of BA produced loose callus and the differentiation was late. On the medium of MS + BA22.2-44.4μM + IAA 1-5μM, Hypocotyl segments can be differentiated directly into stems; in hormone-free MS medium, hypocotyl segments can be directly seeded; loose callus is a good material for suspension culture and can be used for protoplast culture and anti- Clone screening.