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为了丰富马铃薯分子标记,定位青枯病抗性基因位点,利用具有抗青枯病遗传背景的二倍体马铃薯亲本USW5337.3(C)和772102.37(E)及其123份杂交一代无性系,进行了马铃薯SSR遗传图谱的构建。根据马铃薯全基因组序列设计了864对SSR引物,其中187对在亲本间表现出差异,135对(72.2%)能够在杂交一代中扩增出清晰的条带,最终构建了一张由12个连锁群组成,包含135个SSR标记的马铃薯分子标记遗传图谱。12个连锁群总长度为948.4 c M,标记间平均间距7.03 c M,含有5个标记偏分离区域。不仅为马铃薯饱和分子遗传图谱的构建提供新的SSR标记,对于马铃薯抗青枯病QTL定位、基因克隆和分子标记辅助选择也具有重要的参考意义。
In order to enrich potato molecular markers and locate bacterial wilt resistance gene loci, diploid potato parents USW 5333.3 (C) and 772102.37 (E) with their genetic background of resistance to bacterial wilt and 123 hybrid clones of the same generation were used, The potato SSR genetic map was constructed. 864 pairs of SSR primers were designed according to the whole genome sequence of potato, of which 187 pairs showed the difference between the parents and 135 pairs (72.2%) were able to amplify clear bands in the hybrid generation. Finally, A cluster consisting of 135 SSR markers of potato molecular marker genetic map. The total length of the 12 linkage groups was 948.4 cM, the average spacing between markers was 7.03 cM, and there were 5 markers with partial segregation. It not only provides a new SSR marker for the construction of molecular map of satiety of potato, but also has important reference significance for QTL mapping, gene cloning and molecular marker-assisted selection of potato bacterial wilt.