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2015年1月珠海出入境检验检疫局首次从鲜葡萄中截获我国进口南非鲜食苹果议定书中关注的检疫性有害生物——南非石竹卷蛾(Epichoristodes acerbella),本文对该虫的寄主、分布及形态特征进行了详细描述。文中应用DNA条形码技术扩增了该虫的线粒体COI基因的部分序列,并与Genbank数据库和DNA条形码数据库BOLDSYSTEMS v3中已知序列比对,结果与Genbank中2条南非石竹卷蛾序列98%以上一致;与BOLDSYSTEMS v3数据库中10条南非石竹卷蛾序列98%以上一致。
In January 2015, Zhuhai CIQ for the first time intercepted the quarantine pest (Epichoristodes acerbella), which is the focus of our country’s import of fresh apples from South Africa, from fresh grapes. The host, distribution and Morphological characteristics were described in detail. The sequence of mitochondrial COI gene was amplified by DNA barcoding and compared with the known sequence in GenBank database and DNA barcoding database BOLDSYSTEMS v3. The result was consistent with that of two S. dorsalis in Genbank ; More than 98% of the sequence of 10 S. dorsalis in BOLDSYSTEMS v3 database.