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目的:考察甘草酸单铵(MAG)对异烟肼(INH)诱导的大鼠肝损伤的保护作用及其对肝脏转运体Na+-牛磺胆酸共转运多肽(Na+-taurocholate cotransporting polypeptide,NTCP)及多药耐药相关蛋白2(multidrug resistance protein 2,Mrp2)的调节作用。方法:Wistar雄性大鼠45只,随机分为9组,即对照组,异烟肼肝损伤组(INH组),甘草酸单铵治疗组(MAG组),以上每组又各分为干预7,14,21 d组。INH组大鼠灌胃INH60 mg·kg-1·d-1;MAG组大鼠灌胃MAG45 mg·kg-1·d-1 3 h后灌胃INH。干预结束后,处死大鼠,通过血清生化指标、肝脏病理及Ntcp、Mrp2蛋白表达对各组大鼠进行评价对比。结果:血清生化指标与肝脏病理结果显示,INH诱导的大鼠肝损伤具有时间依赖性,而MAG对INH诱导的大鼠肝损伤具有保护作用,且存在时间依赖性;与INH组相比,MAG干预21 d对肝脏转运体Ntcp及Mrp2表达具有明显下调作用(P<0.05)。结论:MAG对INH诱导的肝损伤的保护作用可能与下调肝细胞膜转运体Ntcp与Mrp2的表达有关。
AIM: To investigate the protective effect of monoammonium glycyrrhizinate (MAG) on liver injury induced by isoniazid (INH) in rats and its effect on the expression of Na + -taurocholate cotransporting polypeptide (NTCP) And multidrug resistance protein 2 (Mrp2) regulation. Methods: Forty-five Wistar male rats were randomly divided into 9 groups: control group, isoniazid-induced liver injury group (INH group) and monoammonium glycyrrhizinate group (MAG group), and each group was divided into intervention group 7 , 14,21 d group. The rats in INH group were given intragastric INH 60 mg · kg -1 · d-1, while MAG rats were intragastrically infused with MAG 45 mg · kg -1 · d-1 for 3 h. After the intervention, the rats were sacrificed and the rats in each group were evaluated and compared by the serum biochemical indexes, liver pathology and Ntcp, Mrp2 protein expression. RESULTS: Serum biochemical markers and liver pathology showed that INH-induced hepatic injury was time-dependent, while MAG had a protective effect on INH-induced hepatic injury in rats and was time-dependent. Compared with INH group, MAG Intervening for 21 d significantly reduced the expression of Ntcp and Mrp2 in liver transporters (P <0.05). CONCLUSION: The protective effect of MAG on INH-induced liver injury may be related to the down-regulation of Ntcp and Mrp2 expression in liver cell membrane transporters.