论文部分内容阅读
AIM:To set up a new method to detect tissue inhibitors ofmetalloproteinase-1 and-2(TIMP-1 and TIMP-2)in sera ofpatients with hepatic cirrhosis,and to investigate theexpression and location of TIMP-1 and TIMP-2 in liver tissueof patients with hepatic cirrhosis,and the correlationbetween TIMPs in liver and those in sera so as to discusswhether TIMPs can be used as a diagnosis index of hepaticfibrosis.METHODS:The monoclonal antibodies(McAbs)of TIMP-1and TIMP-2 were used to sensitize erythrocytes,and solid-phase absorption to sensitized erythrocytes(SPASE)wasused to detect TIMP-1 and TIMP-2 in the sera of patients withhepatic cirrhosis.Meanwhile,with the method of in situhybridization and immunohistochemistry,we studied themRNA expression and antigen location of TIMP-1 and TIMP-2in the livers of 40 hepatic cirrhosis patients with pathologicdiagnosis.RESULTS:With SPASE,they were 16.4 % higher in theacute hepatitis group,33.3 % higher in the chronic hepatitisgroup,and the positive rates were 73.6 % and 61.2 %respectively in sera of hepatic cirrhosis patients,which wereremarkably higher than those in chronic hepatitis and acutehepatitis group(P<0.001).In 40 samples of hepaticcirrhosis tissues,all of them showed positive expression ofTIMP-1 and TIMP-2 mRNA detected withimmunohistochemistry or in situ hybridization(positive ratewas 100 %).Expression of TIMPs in different degrees couldbe found in liver tissue with cirrhosis.TIMPs were located incytoplasm of liver cells of patients with hepatic cirrhosis.There was a significant correlation between serum TIMPslevel and liver TIMPs level.CONCLUSION: SPASE is a useful method to detect the TIMP I and TTMP-2 in sera of patients with hepatic cirrhosis, and TIMP-1 and TIMP-2 can be considered as a useful diagnostic index of hepatic fibrosis. especially TIMP-1.
AIM: To set up a new method to detect tissue inhibitors of metalloproteinase-1 and-2 (TIMP-1 and TIMP-2) in sera of patients with hepatic cirrhosis, and to investigate theexpression and location of TIMP-1 and TIMP-2 in liver tissueof patients with hepatic cirrhosis, and the correlation between TIMPs in liver and those in sera so as to discuss whether TIMPs can be used as a diagnosis index of hepatic fibrosis. METHODS: The monoclonal antibodies (McAbs) of TIMP-1 and TIMP-2 were used to sensitize erythrocytes, and solid-phase absorption to sensitized erythrocytes (SPASE) was used to detect TIMP-1 and TIMP-2 in the sera of patients with hepatic cirrhosis. Meanwhile while with the method of in situ hybridization and immunohistochemistry, we studied theyRNA expression and antigen location of TIMP-1 and TIMP-2 in the livers of 40 hepatic cirrhosis patients with pathologic diagnosis of high hepatic disease, with a positive rate were 33.4% higher in the chronic hepatitis group 73.6% and 61.2% respectively in sera of hepatic cirrhosis patients, which were significantly higher than those in chronic hepatitis and acute liver disease groups (P <0.001) .In 40 samples of hepaticcirrhosis tissues, all of them showed positive expression of TIMP-I and TIMP-2 mRNA detected with immunohistochemistry or in situ hybridization (positive rate was 100%). Expression of TIMPs in different degrees could be found in liver tissue with cirrhosis. TIMPs were located in hepatic placenta of liver cells of patients with hepatic cirrhosis. There was a significant correlation between serum TIMPslevel and liver TIMPs level. CONCLUSION: SPASE is a useful method to detect the TIMP I and TTMP-2 in sera of patients with hepatic cirrhosis, and TIMP-1 and TIMP-2 can be considered as a useful diagnostic index of hepatic fibrosis. especially TIMP -1.