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干细胞因子 (stemcellfactor ,SCF)是一种对造血细胞有重要作用的因子 ,在自体外周血造血前体细胞移植、肿瘤放化疗的辅助治疗、贫血和其他血液病的治疗方面有良好的应用前景。目的 :分析本所制备的rhSCF的理化性质和生物活性。方法 :用反相高效液相色谱、质谱、凝胶高效液相色谱等方法分别测定了制品的纯度、分子量、氨基酸组成、紫外光谱等 ,并用TF 1细胞测定了制品的生物活性。结论 :制品分子量为 18558,与天然hSCF的差异小于 0 .2 % ,产物以非共价二聚体形式存在。氨基酸组成与天然hSCF相同 ,紫外光谱为典型的蛋白光谱 ,在 2 77nm有特征吸收 ,比活为 0 .95× 10 6U /mg ,与国外参考品相似。上述结果初步说明制备的rhSCF理化性质与天然hSCF一致 ,生物活性良好 ,有望进一步开发用于临床。
Stem cell factor (SCF) is a factor that plays an important role in hematopoietic cells. It has good application prospect in autologous peripheral blood hematopoietic cell transplantation, adjuvant chemotherapy of tumor radiotherapy and chemotherapy, anemia and other hematological diseases. Objective: To analyze the physicochemical properties and biological activity of rhSCF. Methods: Purity, molecular weight, amino acid composition and UV spectrum of the product were determined by RP-HPLC, MS and gel-HPLC respectively. The biological activity of the product was measured by TF1 cells. Conclusion: The molecular weight of the product is 18558, which is less than 0.2% of that of natural hSCF. The product exists as non-covalent dimer. The amino acid composition is the same as that of natural hSCF. The UV spectrum is a typical protein spectrum with a characteristic absorption at 2 77 nm. The specific activity is 0.95 × 10 6 U / mg, which is similar to foreign references. The above results preliminary shows that the prepared rhSCF physical and chemical properties consistent with the natural hSCF, good biological activity, is expected to be further developed for clinical use.