论文部分内容阅读
目的:观察加味聚精食疗方对少、弱精子症小鼠睾丸组织中CR16表达的影响。方法:60只8周龄雄性小鼠适应性饲养1周后随机编号,采取区组随机化法将小鼠分为6组:正常组(G1)、DS模型空白对照组(G2)、低剂量治疗组(G3)、中剂量治疗组(G4)、高剂量治疗组(G5)、预防治疗组(G6),每组10只。G2~G6组通过尾静脉单剂量注射5-FU(100 mg/kg),14 d后形成稳定的DS模型。G1正常组全程给予蒸馏水4 g/kg灌胃,连续40 d;G2空白对照组及G3~G5治疗组自造模开始即给予蒸馏水4 g/kg灌胃,G6预防治疗组给予加味聚精食疗方6.3 g/kg灌胃,连续14 d。G2~G6组造模完成后,G2组予蒸馏水4 g/kg灌胃;G3~G5组分别予加味聚精食疗方低剂量(3.15 g/kg)灌胃、中剂量(6.3 g/kg)灌胃、高剂量(12.6 g/kg)灌胃;G6组继续蒸馏水4 g/kg连续灌胃。以上均1次/d,连续喂养26 d。各组治疗结束后作集中处死,计算机辅助精子分析(CASA)技术检测小鼠附睾精子质量;对小鼠肝、肾器官及睾丸组织进行病理学检查;免疫组织化学S-P法检测基因CR16在SD动物模型体内的表达。结果:在精子质量的改善方面,G2、G6组比较无明显差异,不具有统计学意义(P>0.05)。G3、G4、G5组均较G2、G6组有明显改善(P<0.05),且G4组优于G3、G5组(P<0.05)。在阴性对照无特异性着色的情况下对结果进行判读,G3、G4、G5组小鼠睾丸组织生精小管上皮中CR16均有不同程度的表达,明显高于G2、G6组,差异具有统计学意义(P>0.05),G4组小鼠睾丸组织结构修复情况、睾丸组织CR16的表达水平(染色程度)均明显上调。镜下观察见小鼠睾丸组织中CR16阳性反应呈棕褐色或浅棕色颗粒状,散在分布于支持细胞胞浆胞膜。结论:加味聚精食疗方能够一定程度上提高附睾精子的质量,安全无不良反应,其通过上调DS动物模型体内CR16的表达水平可能是治疗少、弱精子症的作用机制之一。
Objective: To observe the effects of Jiaweifujiangyan Recipe on the expression of CR16 in the testes of mice with asthenospermia. Methods Sixty male, 8-week-old mice were randomly fed one week after induction. The mice were randomly divided into 6 groups: normal group (G1), DS model blank control group (G2), low dose Treatment group (G3), middle dose treatment group (G4), high dose treatment group (G5), prevention treatment group (G6), each group of 10. Groups G2-G6 received single-dose injection of 5-FU (100 mg / kg) via the tail vein and formed a stable DS model after 14 days. G1 normal group given distilled water 4 g / kg gavage for 40 days; G2 blank control group and G3 ~ G5 treatment group from the beginning of modeling that is given distilled water 4 g / kg gavage, G6 prophylaxis treatment group was given concentrated concentrating diet Party 6.3 g / kg gavage for 14 days. Group G2 was given gavage with distilled water 4 g / kg orally in group G2, and group G3 G5 was given low dose (3.15 g / kg) Gavage, high-dose (12.6 g / kg) gavage; G6 group continued to distilled water 4 g / kg continuous gavage. The above were 1 / d, continuous feeding 26 d. The rats in each group were sacrificed at the end of the treatment, the epididymal sperm quality was detected by computer assisted sperm analysis (CASA) technique, pathological examination was performed on the liver and kidney organs and testis tissue of mice, and immunohistochemistry SP method was used to detect the expression of CR16 gene in SD animals Model body expression. Results: There was no significant difference in the improvement of sperm quality between G2 and G6 groups (P> 0.05). The G3, G4 and G5 groups were significantly improved compared with the G2 and G6 groups (P <0.05), and the G4 group was superior to the G3 and G5 groups (P <0.05). Interpretation of the results in the absence of specific staining of the negative control, CR3 in seminiferous tubules of G3, G4, G5 mice testicular tissue were different levels of expression, significantly higher than G2, G6 group, the difference was statistically significant (P> 0.05). The histological structure of testis in G4 group and the expression of CR16 in testis tissue were significantly up-regulated. Microscopically observed CR16 positive reaction in mouse testis was tan or light brown granular, scattered in the cytoplasmic membrane supporting cells. CONCLUSION: The flavored concentrates can improve the quality of epididymal sperm to a certain degree without any adverse reactions. It may be one of the mechanisms of treatment of oligozoospermia by up-regulating the expression of CR16 in DS animal models.