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目的研究RhoC基因对胆管癌QBC939细胞株的增殖及cyclinD1 mRNA表达的影响。方法将正、反义RhoC cDNA真核表达载体,转染人胆管癌细胞QBC939,检测细胞生长曲线,RT-PCR检测cyclinD1在QBC939细胞、转染空载体的QBC939细胞(QBC939-V)、转染正义RhoC的QBC939细胞(QBC939-S)、转染反义RhoC的QBC939细胞(QBC939-AS)的mRNA相对表达量。结果与QBC939及QBC939-V对照细胞相比,QBC939-S体外生长较快,QBC939-AS体外生长较慢,cyclinD1表达在QBC939-AS与对照组减少(P<0.05),cyclinD1表达在QBC939-S与对照组增加(P<0.05)。结论 RhoC可能通过调节cyclinD1来调控QBC939的增殖能力。
Objective To investigate the effect of RhoC gene on the proliferation and expression of cyclinD1 mRNA in human cholangiocarcinoma QBC939 cell line. Methods The positive and negative RhoC cDNA eukaryotic expression vector was transfected into human cholangiocarcinoma cell line QBC939 to detect the cell growth curve. The expression of cyclinD1 in QBC939 cells was detected by RT-PCR and transfected into empty vector QBC939-V cells (QBC939-V) QBC939 cells (QBC939-S) for sense RhoC, mRNA relative expression of QBC939 cells transfected with antisense RhoC (QBC939-AS). Results Compared with QBC939 and QBC939-V control cells, QBC939-S grew faster in vitro, the growth of QBC939-AS was slower in vitro, the expression of cyclinD1 decreased in QBC939-AS and the control group (P <0.05), and the expression of cyclinD1 in QBC939-S And control group increased (P <0.05). Conclusion RhoC may regulate the proliferation of QBC939 by regulating cyclinD1.