论文部分内容阅读
目的探讨乙型肝炎病毒DNA(HBV-DNA)病毒载量与乙型肝炎血清标志物(HBV-M)之间的关系。方法运用酶联免疫吸附测定法、荧光定量聚合酶链反应(PCR)技术检测857例乙型肝炎患者的HBV-DNA含量和血清学标志物(两对半)。结果血清学检测乙型肝炎表面抗原(HBsAg)+乙型肝炎病毒e抗原(HBeAg)+乙型肝炎病毒核心抗体(抗-HBc)阳性(大三阳)患者HBV-DNA阳性率明显高于其他类型(阳性率97.9%),且其病毒载量显著高于其他组。HBsAg+乙型肝炎病毒e抗体(HBeAb)+抗HBc阳性(小三阳)患者阳HBV-DNA性检出率也较高(阳性率51.3%),其病毒载量高于其他组。结论 HBV-DNA与HBeAg的存在明显正相关。在临床实践中,不仅要用HBV-M,来判断HBV是否在体内复制,还要结合PCR检测技术来测定HBV-DNA含量。
Objective To investigate the relationship between hepatitis B virus DNA (HBV-DNA) viral load and hepatitis B serum marker (HBV-M). Methods Enzyme-linked immunosorbent assay and fluorescent quantitative polymerase chain reaction (PCR) were used to detect HBV-DNA levels and serological markers in 857 patients with hepatitis B (two-and-a-half pairs). Results Serological tests showed that the positive rate of HBV-DNA in patients with HBsAg + HBeAg + HBeAg (anti-HBc) was significantly higher than that in other patients Type (positive rate of 97.9%), and its viral load was significantly higher than the other groups. HBsAg + hepatitis B virus e antibody (HBeAb) + anti-HBc positive (small three positive) positive HBV-DNA detection rate (positive rate 51.3%), the viral load higher than the other groups. Conclusion HBV-DNA is positively correlated with the presence of HBeAg. In clinical practice, not only to use HBV-M, to determine whether HBV replication in vivo, but also combined with PCR detection of HBV-DNA content.