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目的观察制马钱子对佐剂性关节炎(adjuvant arthritis,AA)模型大鼠免疫调节的影响。方法将大鼠随机分为空白对照组、模型组、雷公藤多苷组、制马钱子低剂量组、制马钱子高剂量组,除空白对照组外,每鼠右后趾注射弗氏完全佐剂(CFA)致炎,诱发AA。致炎后,各组分别予相应药物干预,1个月后观察大鼠足趾关节病理变化,并检测血清白细胞介素-1(IL-1)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)水平。结果各药物组均可改善关节滑膜增生和炎性细胞浸润,其中制马钱子高剂量组作用更为明显。模型组血清IL-1、IL-6、TNF-α水平均显著升高(P<0.05,P<0.01),而各药物组IL-1、IL-6、TNF-α水平均有明显降低(P<0.05或P<0.01),且在调节IL-6、TNF-α水平方面制马钱子两个剂量组均明显优于雷公藤多苷组(P<0.05)。结论制马钱子可以明显降低由CFA引起的细胞因子升高。
Objective To observe the effect of Strychnine on immune regulation in adjuvant arthritis (AA) model rats. Methods Rats were randomly divided into blank control group, model group, Tripterygium glycosides group, system of low dose of Strychnine group, and high dose group of Strychnos subtraction system. In addition to the blank control group, rats were injected with Freund’s right posterior toe Complete adjuvant (CFA) causes inflammation and induces AA. After the inflammation, each group was given the corresponding drug intervention. After one month, the pathological changes of the toe joints of the rats were observed, and serum interleukin-1 (IL-1) and interleukin-6 (IL-6) were detected. Tumor necrosis factor-alpha (TNF-alpha) levels. Results All drugs could improve the synovial hyperplasia and inflammatory cell infiltration, and the effect of high-dose group was more obvious. The levels of serum IL-1, IL-6 and TNF-α in the model group were significantly increased (P<0.05, P<0.01), while the levels of IL-1, IL-6 and TNF-α in the drug group were significantly decreased ( P<0.05 or P<0.01), and in the regulation of IL-6, TNF-α levels in the system of two doses of Strychnos subgroup were significantly better than Tripterygium glucosides group (P <0.05). Conclusion Strychnine can significantly reduce the increase of cytokines caused by CFA.