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以黄瓜‘中农19号’为材料,研究了温室无土栽培条件下水肥耦合对几种氮代谢酶及相关物质含量的影响。设3种单株灌水量水平:87L(W1)、125L(W2)、162L(W3),3种单株施肥量水平:64g(F1)、92g(F2)、119g(F3)。试验结果表明,灌水量处理对植株叶片硝酸还原酶(NR)、谷氨酰胺合成酶(GS)、谷氨酸合成酶(GOGAT)、谷氨酸脱氢酶(GDH)活性及硝态氮和可溶性蛋白质含量的正影响显著,在F1施肥水平下,W1的几种氮代谢指标高于W2和W3;在F2和F3施肥水平下,W2的灌水量水平最好。施肥量处理对NR、GS、GOGAT、GDH活性及硝态氮和可溶性蛋白质含量的正影响皆达到极显著水平,在相同灌水量水平下,F3>F2>F1。灌水量和施肥量2因素之间有显著的互作效应。从相关分析上看氮代谢酶活性及相关物质含量与黄瓜产量及果实中硝酸盐、游离氨基酸、可溶性蛋白质及可溶性糖含量之间呈显著的正相关。
Cucumber ’Zhongnong 19’ was used to study the effects of water and fertilizer coupling on the activities of several nitrogen metabolism enzymes and related substances in greenhouse under soilless culture conditions. Three irrigation levels were set as follows: 87L (W1), 125L (W2) and 162L (W3). The fertilization levels of three plants were 64g (F1), 92g (F2) and 119g (F3) respectively. The results showed that the effects of irrigation amount on the activities of nitrate reductase (NR), glutamine synthetase (GS), glutamate synthase (GOGAT), glutamate dehydrogenase (GDH) The positive effect of soluble protein content was significant. Under the F1 fertilization level, several nitrogen metabolism indices of W1 were higher than those of W2 and W3. Under the F2 and F3 fertilization levels, the W2 irrigation level was the best. The positive effects of fertilization on the activities of NR, GS, GOGAT, GDH and the content of nitrate nitrogen and soluble protein all reached the extremely significant level. Under the same irrigation level, F3> F2> F1. There was a significant interaction effect between irrigation amount and fertilizer amount. Correlation analysis indicated that there was a significant positive correlation between nitrogen metabolism enzyme activity and related substances and the contents of nitrate, free amino acids, soluble protein and soluble sugar in cucumber yield and fruit.