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目的：观察抗VEGF抗体及抗flt抗体对HEP－2人喉癌细胞生长的影响，探讨VEGF对HEP－2细胞的作用及作用方式。方法：将不同浓度的抗VEGF及抗flt抗体分别加入HEP－2细胞的培养液中，培养5天后测HEP－2细胞的活性（MTT法）。结果：不同浓度的VEGF抗体作用于HEP－2细胞时，浓度从10μg／ml至1μg／ml时，对HEP－2细胞的生长具有显著的抑制作用（P＜0．01），当VEGF浓度低于1μg／ml时，无明显抑制作用；抗flt抗体浓度从10μg／ml减至0．1μg／ml时，对HEP－2细胞的生长均有显著抑制作用（P＜0．01），当浓度低于0．1μg／ml时，抗flt抗体对HEP－2细胞的生长无显著抑制作用（P＜0．05）。结论：在体外培养条件下，中和VEGF抗体及封闭VEGF的受体flt对人喉癌HEP－2细胞的生长均有显著抑制作用，显示VEGF作为一种自分泌和旁分泌生长因子在喉癌的发生发展中发挥重要作用。
OBJECTIVE: To observe the effect of anti-VEGF antibody and anti-flt antibody on the growth of HEP-2 human laryngeal carcinoma cells and to explore the effect of VEGF on HEP-2 cells and the mode of action. Methods: Different concentrations of anti-VEGF and anti-flt antibody were added into the culture medium of HEP-2 cells, and the activity of HEP-2 cells was measured after 5 days culture (MTT method). Results: Different concentrations of VEGF antibody significantly inhibited the growth of HEP-2 cells when treated with HEP-2 at a concentration of 10μg / ml to 1μg / ml (P <0.01). When VEGF concentration was low At 1μg / ml, there was no significant inhibitory effect. When the concentration of anti-flt antibody was reduced from 10μg / ml to 0.1μg / ml, the growth of HEP-2 cells was significantly inhibited (P <0.01) When the concentration was lower than 0.1μg / ml, anti-flt antibody had no significant inhibitory effect on the growth of HEP-2 cells (P <0.05). CONCLUSION: Under the conditions of in vitro culture, neutralizing VEGF antibody and blocking VEGF receptor flt significantly inhibited the growth of human laryngeal carcinoma HEP-2 cells, indicating that VEGF as an autocrine and paracrine growth factor in laryngeal cancer Play an important role in the occurrence and development.