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通过紫外(ultraviolet,UV)光谱和圆二色(circulardichroism,CD)光谱,研究了丁基锡化合物(一丁基锡、二丁基锡和三丁基锡)与脱氧核糖核酸(deoxyribonucleicacid,DNA)的作用方式以及时间和浓度的影响。结果显示丁基锡化合物与DNA的作用是双重的,既作用于DNA的碱基,对双螺旋结构有一定影响,又作用于DNA的磷酸基团,使构象发生变化。但是,丁基锡化合物与脱氧核糖核酸作用的程度和方式与丁基锡种类、时间和浓度等因素有关。一丁基锡倾向于与磷酸基团作用,三丁基锡倾向于与碱基作用,而二丁基锡与两者作用程度相近。短时间内,丁基锡化合物的作用位点通常是DNA的碱基;长时间时,则作用位点往往是DNA的磷酸基团。低浓度的丁基锡化合物倾向与DNA的碱基结合,高浓度的丁基锡化合物倾向与DNA的磷酸基团结合。
The mode of action of butyltin compounds (monobutyltin, dibutyltin and tributyltin) and deoxyribonucleic acid (DNA) as well as the effects of time and concentration on the DNA were studied by ultraviolet (UV) spectroscopy and circular dichroism spectroscopy influences. The results showed that the interaction between butyltin compounds and DNA was twofold, which not only acted on the base of DNA, but also affected the double helix structure and also acted on the phosphate group of DNA to change the conformation. However, the extent and manner in which butyltin compounds interact with DNA are related to the type, time and concentration of butyltin. Butyltin tends to interact with phosphate groups, and tributyltin tends to interact with the base, whereas dibutyltin has a similar degree of interaction with both. For a short period of time, the site of action of butyltin compounds is usually the base of DNA; for a long time, the site of action is often the phosphate group of DNA. Low concentrations of butyltin compounds tend to bind to the bases of DNA, and high concentrations of butyltin compounds tend to bind to the phosphate groups of DNA.