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用烟草花蕾观察研究烟草花粉母细胞减数分裂,对于鉴定和分析烟草核型以及染色体在减数分裂过程中的行为,是一种有价值的实验方法。现将观察方法和我们工作中的点滴体会介绍于后。一、取材取材包括所采花蕾的大小、取样的时间和取样时田间温度。而花蕾的大小则是观察减数分裂的关键。据我们6月4日以晾晒烟(黄花烟草,2n=48)为材料,从7时半到18时半(夏时制),每隔1小时取样同时测定田间温度,对12个样品进行观察的结果表明,
Observing the meiosis of tobacco pollen mother cells with tobacco flower buds is a valuable experimental method for identifying and analyzing the tobacco karyotype and the behavior of chromosomes during meiosis. Now the observation method and the bit by bit in our work experience introduced later. First, drawn from including the size of the buds taken, sampling time and field temperature sampling. The size of buds is the key to observe meiosis. According to our data, on June 4, from the time of drying tobacco (yellow tobacco, 2n = 48), from 7:30 to 18:30 (summer time), samples were taken every 1 hour and the field temperature was measured at the same time. Twelve samples were observed The results show that,