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目的研究牛磺酸对锰致大鼠海马线粒体超微结构改变及氧化损伤的保护作用。方法 W istar大鼠80只随机分为对照组、染锰组、牛磺酸预防组、牛磺酸治疗组,连续染毒12周后制备海马线粒体,电镜扫描超微超微结构变化,测定线粒体活性氧簇(ROS)变化;体外制备成年健康大鼠的海马线粒体并与锰、牛磺酸孵育,观察ROS变化。结果染锰致线粒体空泡化、嵴消失,牛磺酸可改善线粒体的超微结构损伤;体内染锰后染锰组海马线粒体ROS为(170.1±6.1),明显低于对照组(182.2±5.5)和牛磺酸预防组(207.0±6.0),高于牛磺酸治疗组(150.2±3.8),差异均有统计学意义(t=3.63,t=11.61,t=6.31,P<0.05);线粒体体外染锰后染锰组ROS为(2 139.2±24.9),明显高于对照组(650.7±12.0)、牛磺酸预防组(1 734.5±20.7)和牛磺酸治疗组(1 477.3±14.8),差异均有统计学意义(t=137.23,t=37.31,t=61.01,P<0.05)。结论牛磺酸可改善染锰损伤的大鼠海马线粒体超微结构;体外实验结果表明,用牛磺酸预防和治疗染锰大鼠均能明显降低其ROS。
Objective To study the protective effect of taurine on the ultrastructure of mitochondria and oxidative damage induced by manganese in rats. Methods Eighty Wistar rats were randomly divided into control group, manganese group, taurine prevention group and taurine treatment group. After continuous exposure for 12 weeks, the hippocampal mitochondria were prepared and their ultrastructural changes were observed by scanning electron microscopy. Reactive oxygen species (ROS) in hippocampus of hippocampus. The mitochondria of hippocampus were prepared in vitro and incubated with manganese and taurine to observe the changes of ROS. Results The mitochondria of Mn-killed mitochondria were disappeared and taurine disappeared. The ultrastructural damage of mitochondria was improved by taurine. The mitochondrial ROS of hippocampus of Mn-dyed group after manganese exposure was (170.1 ± 6.1), which was significantly lower than that of control group (182.2 ± 5.5 (T = 3.63, t = 11.61, t = 6.31, P <0.05); and mitochondria in the taurine prevention group (207.0 ± 6.0) were significantly higher than those in the taurine treatment group (150.2 ± 3.8) The level of ROS in the manganese-treated group was (2 139.2 ± 24.9), which was significantly higher than that in the control group (650.7 ± 12.0), taurine prevention group (1734.5 ± 20.7) and taurine treatment group (1477.3 ± 14.8) The differences were statistically significant (t = 137.23, t = 37.31, t = 61.01, P <0.05). Conclusion Taurine can improve the ultrastructure of mitochondria in hippocampus of manganese-damaged rats. In vitro experiments showed that taurine could significantly reduce the ROS of rats exposed to manganese.