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应用人血在体外培养,分别用PWM和LPS诱导淋巴细胞,接受各种剂量~(60)Coγ线照射,两种细胞按各种组合混合再培养,并设有各种单项培养作对照,以~3H-TdR掺入反映淋巴细胞的增殖转化。实验结果说明PWM细胞能够激活LPS细胞转化,当一种细胞受照后单独培养或与另一种正常细胞混合培养,其放射性掺入即下降,并随剂量增加而愈益明显、下降趋势均呈现负相关,并分别得到直线回归方程,差异显著性比较说明PWM细胞受照影响更严重。当PWM细胞接受1Gy以上剂量后与正常LPS细胞混合培养即失去激活作用,LPS细胞受照1—2 Gy仍然可被激活,以上提示电离辐射导致机体免疫缺陷,其中PWM诱导的T辅助细胞受损是较重要的。
Human blood was cultured in vitro and lymphocytes were induced with PWM and LPS, respectively, and irradiated with various doses of 60 Co γ rays. The two cells were mixed and cultured in various combinations, and various single cultures were used as controls. ~3H-TdR incorporation reflects the proliferation and transformation of lymphocytes. The experimental results show that the PWM cells can activate LPS cell transformation. When a cell is irradiated alone or mixed with another normal cell, its radioactive incorporation decreases, and it becomes more obvious as the dose increases, and the decreasing trend is negative. Relevant and linear regression equations were obtained respectively. The comparison of the significance of the differences indicates that the effects of the exposure of the PWM cells are more serious. When PWM cells receive more than 1 Gy dose, they lose their activation when mixed with normal LPS cells. LPS cells can still be activated when they are exposed to 1-2 Gy. These results suggest that ionizing radiation causes immune deficiency, and PWM-induced T helper cells are damaged. It is more important.