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通过电转化方法将含球形芽孢杆菌（Bacillussphaericus简称B.s)二元毒素基因的重组质粒pCW2转化野生型苏云金杆菌以色列亚种（B．thuringiensissubsp.israelensis简称B．t．i），获得一株含二元毒素基因的重组菌株B＋CW-1。重组菌株能表达二元毒素及δ-内毒素和CytA毒素，并形成2类位于芽孢孢外膜外的伴孢晶体。B＋CW-1对伊蚊（Aedes）、按蚊（Anopheles）和抗性库蚊（Culex）的毒力明显高于B．s，同时也略高产B.t.i菌株，但其杀蚊谱和杀活性水平同B．t．i相似。未发现二元毒素同B．t．i毒素间的协同作用。
The recombinant plasmid pCW2 containing Bacillus subtilis (Bs) binary toxin gene was transformed into B. thuringiensis subsp. Israelensis (B.t.i.) by electrotransformation, Recombinant strain B + CW-1 of the toxin gene. The recombinant strain can express binary toxin and delta-endotoxin and CytA toxin, and form two types of conidia crystals located outside the outer spore of sporulation. The toxicity of B + CW-1 to Aedes, Anopheles and Culex was significantly higher than that of B + CW-1. s, slightly higher yield B.t.i strain, but the mosquito spectrum and kill activity with the same level B. t. i is similar. Binary toxin B was not found. t. i toxin synergy between.