论文部分内容阅读
目的：探讨１７ｐＬＯＨ与晚期大肠癌的关系。方法：运用ＳｏｕｔｈｅｒｎＢｌｏｔ和ＲＦＬＰ（ｒｅｓｔｒｉｃｔｉｏｎｆｒａｇｍｅｎｔｌｅｎｇｔｈｐｏｌｙｍｏｒ－ｐｈｉｓｍａｎａｌｙｓｉｓ）法进行分析。结果：１０９例大肠癌的ＲＦＬＰ分析显示，１７ｐＬＯＨ的频率在ＤｕｋｅｓＤ期（９５％）高于ＤｕｋｅｓＡ、Ｂ、Ｃ各期（５４％～６８％），Ｐ＝０．００６；１７ｐＬＯＨ频率在大肠癌远处器官转移组（９５％）高于大肠癌不伴远处器官转移组（Ｐ＝０．００１）；在大肠癌淋巴结转移组中１７ｐＬＯＨ的频率（７１％），与无淋巴结转移组（６４％）相比，两者无显著差异（Ｐ＞０．０５）。结论：１７ｐＬＯＨ是晚期大肠癌的重要基因标记，并提示临床预后不佳。
Objective: To investigate the relationship between 17pLOH and advanced colorectal cancer. Methods: SouthernBlot and RFLP (restrictionfragmentlengthpolymor-phismanalysis) methods were used for analysis. RESULTS: RFLP analysis of 109 cases of colorectal cancer revealed that the frequency of 17pLOH was higher in DukesD (95%) than in Dukes A, B, and C (54% to 68%), P = 0.006; 17pLOH frequency was significantly higher in colorectal cancer. The organ metastasis group (95%) was higher than the colorectal cancer group without distant organ metastasis (P=0.001); the frequency of 17pLOH in the lymph node metastasis group (71%), and no lymph node metastasis group (64%) There was no significant difference between the two (P>0.05). Conclusion: 17pLOH is an important gene marker for advanced colorectal cancer, and it suggests that the clinical prognosis is poor.