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To evaluate the effect of lipopolysaccharide (LPS) on the expression of tumor necrosis factor α (TNFα ) and interleukin- 8 (IL- 8) protein in endometriotic stromal cells (ESC) and their effect on the proliferation of ESC. Prospective study. Department of Obstetrics and Gynecology, Tottori University Hospital, Yonago, Japan. Seventeen patients who underwent laparoscopic surgery. Endometriotic stromal cells were obtained from chocolate cyst linings of the ovary. We determined the effect of LPS on the production of TNFα .and IL- 8 and the effect of IL- 8 antisense oligonucleotide and nuclear factor κ B(NF κ B) inhibitor on IL- 8 production using ELISA. TNFα production was examined by immunocytochemical staining. We determined the effect of LPS and the effect of IL- 8 antisense oligonucleotide and NF κ B inhibitor on LPS promoted ESC proliferation. LPS stimulated ESC produced significant amounts of TNFα .and IL- 8 in a dose and time dependent fashion. Adding LPS promoted ESC proliferation. Anti TNFα .antibody and anti IL- 8 antibody inhibited the stimulatory effects of LPS. IL- 8 antisense oligonucleotide and NF κ B inhibitor significantly decreased LPS induced IL- 8 protein production and LPS induced ESC proliferation. Pelvic inflammation may promote the progression of endometriosis.
To evaluate the effect of lipopolysaccharide (LPS) on the expression of tumor necrosis factor α (TNFα) and interleukin-8 (IL-8) protein in endometriotic stromal cells (ESC) and their effect on the proliferation of ESC. Prospective study. Department of Obstetrics and Gynecology, Tottori University Hospital, Yonago, Japan. Seventeen patients who underwent laparoscopic surgery. Endometriotic stromal cells were obtained from chocolate cyst linings of the ovary. We determined the effect of LPS on the production of TNFα. and IL-8 and the effect of IL-8 antisense oligonucleotide and nuclear factor κ B (NF κ B) inhibitor on IL-8 production using ELISA. TNFα production was examined by immunocytochemical staining. We determined the effect of LPS and the effect of IL- 8 antisense oligonucleotide and NF κ B inhibitor on LPS promoted ESC proliferation. LPS stimulated ESC produced significant amounts of TNFα. and IL-8 in a dose and time dependent fashion. Adding LPS promo ted ESC proliferation. Anti TNFα. Antibody and anti IL-8 antibody inhibited the stimulatory effects of LPS. IL-8 antisense oligonucleotide and NF κ B inhibitor significantly decreased LPS induced IL-8 protein production and LPS induced ESC proliferation. the progression of endometriosis