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目的 探讨茶多酚 (TP)减轻环孢素A(CsA)急性肾毒性的作用及其机制。方法 按照分组 ,分别给予大鼠橄榄油、TP、CsA及TP +CsA ,给药 14d后 ,取血测尿素氮及血、尿肌酐 ,计算肌酐清除率 ,以病理评分法记录肾脏损害程度 ,硫代巴比妥酸法 (TBA法 )测定大鼠肾组织的脂质过氧化水平 ,黄嘌呤氧化法测定肾组织超氧化物歧化酶 (SOD)的活力 ,二硫代二硝基苯甲酸 (DTNB)比色法测定谷胱甘肽过氧化物酶 (GSH Px)活力。结果 使用CsA后 14d ,大鼠的肾脏可见明显损害 ,肌酐清除率明显下降 ,组织丙二醛 (MDA)水平升高 ,SOD与GSH Px活性下降 ;TP具有减轻CsA肾毒性的作用。结论 茶多酚能有效地减轻CsA所致的大鼠肾脏毒性 ,可能与其清除氧自由基、保护肾组织中SOD及GSH Px活性有关。
Objective To investigate the effect of tea polyphenols (TP) on acute nephrotoxicity induced by cyclosporin A (CsA) and its mechanism. Methods Rats were given olive oil, TP, CsA and TP + CsA respectively. After 14 days of administration, blood urea nitrogen and blood and urine creatinine were measured to calculate the creatinine clearance rate. The pathological score was used to record the extent of renal damage, sulfur The level of lipid peroxidation in rat renal tissue was measured by TBA method. The activity of superoxide dismutase (SOD) in renal tissue was measured by xanthine oxidative method. The activity of dithiobitrobenzoic acid (DTNB) ) Colorimetric method for determination of glutathione peroxidase (GSH Px) activity. Results After 14 days of CsA treatment, the kidneys of rats showed obvious damage, the creatinine clearance rate decreased obviously, MDA level and SOD and GSH Px activities decreased. TP had the effect of reducing CsA nephrotoxicity. Conclusion Tea polyphenols can effectively reduce the renal toxicity induced by CsA in rats, which may be related to its ability to scavenge oxygen free radicals and protect SOD and GSH Px in renal tissues.