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用凝胶电泳法研究了DNA在γ射线作用下链断裂和链交联及保护剂对它的保护效应。DNA样品经电泳、荧光染色、紫外灯下照相后对底片进行光密度分析,最后经计算机数据处理,可以得到其辐照前后的分子量分布变化,并由此得出DNA的链断裂和链交联的定量数据。以此研究了冰冻干燥二元体系中羟基肉桂酸衍生物,在室温下对小牛胸腺DNA的辐射保护效应。测量了DNA与咖啡酸及芥子碱在不同摩尔比下时DNA的链交联和链断裂G值,发现两者对DNA均有明显的辐射保护效应,但咖啡酸的辐射保护效应强于芥子碱。对照对它们的ESR研究,并分析它们的结构异同,初步探讨了两者辐射保护效应强弱的原因。
Gel electrophoresis was used to study the DNA damage and chain cross-linking under the action of γ-rays and the protective effect of the protective agent. After DNA electrophoresis, fluorescence staining and ultraviolet light photography, the optical density of the film was analyzed. Finally, the changes of the molecular weight distribution before and after irradiation were obtained by computer data processing, and the DNA strand breaks and chain cross-links were obtained Of quantitative data. In this study, we studied the radiation protective effect of hydroxycinnamic acid derivatives on calf thymus DNA in the freeze-dried binary system at room temperature. DNA and caffeic acid and sinapine were measured at different molar ratios of DNA chain cross-linking and strand breaks G value and found that both have significant radiation protective effect of DNA, but caffeic acid radiation protective effect stronger than sinapine . Contrast to their ESR research and analysis of their structural similarities and differences between the two radiation protection to explore the reasons for the strength.