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采用同源重组方法构建了野生型p53全长cDNA的重组体腺病毒.通过转导人卵巢癌细胞系SK-OV-3和黑色素瘤细胞系WM-983A,证实腺病毒能介导p53基因进行有效转移;并能显著抑制这两种肿瘤细胞的生长和集落形成能力,生长抑制率分别达到93％和86％.对两种肿瘤细胞裸鼠皮下移植瘤的治疗实验表明,p53能明显抑制肿瘤的生长.流式细胞计数、DNA片段化及TUNEL分析证实,p53可诱导肿瘤细胞凋亡和G1期阻滞.结果显示p53重组体腺病毒是一种有良好前景的肿瘤基因治疗药物.
Recombinant adenovirus of wild-type p53 full-length cDNA was constructed by homologous recombination. It was confirmed that adenovirus could mediate p53 gene transduction through transduction of human ovarian cancer cell line SK-OV-3 and melanoma cell line WM-983A. Effective transfer; and can significantly inhibit the growth and colony formation of these two types of tumor cells, the growth inhibition rate reached 93% and 86%, respectively. The treatment experiment of the two tumor cell subcutaneous xenografts in nude mice showed that p53 can significantly inhibit the tumor The growth, flow cytometry, DNA fragmentation and TUNEL analysis confirmed that p53 can induce tumor cell apoptosis and G1 arrest. The results show that p53 recombinant adenovirus is a promising tumor gene therapy drugs.