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目的研究3种氧化剂对白色假丝酵母菌(CAL)菌丝形成的影响,探讨氧化应激对真菌病原体的抑制作用,为研发新的抗真菌药物提供理论依据。方法 2014年2月将3株CAL标准株和临床分离菌株悬液接种于含不同浓度过氧化氢、二酰胺和甲萘醌的RPMI-1640培养基中,分别于不同时间(0、2、4、6h)计数菌丝生成率,数据采用SPSS 13.0软件进行统计分析。结果 3种氧化剂对CAL菌丝形成均有抑制作用,H2O2、二酰胺和甲萘醌均呈剂量依赖性抑制菌丝生成,H2O2、二酰胺和甲萘醌浓度为30mmol/L时,CAL菌丝生成率开始下降,菌丝生成率分别为67.0%、58.0%、49.0%;在H2O2、二酰胺和甲萘醌处理2h时,CAL菌丝生成率开始下降,生成率分别为73.0%、59.0%、51.0%。结论 3种氧化剂呈浓度和时间依赖性抑制CAL菌丝形成,其中甲萘醌抑制CAL菌丝形成的作用最强,并且标准株菌丝生成率低于临床分离株。
Objective To study the effects of three oxidants on the mycelium formation of Candida albicans (CAL), and to explore the inhibitory effect of oxidative stress on fungal pathogens and to provide a theoretical basis for the development of new antifungal agents. Methods Three strains of CAL standard strains and clinical isolates were inoculated into RPMI-1640 medium containing different concentrations of hydrogen peroxide, diamide and menaquinone in February 2014, respectively, at different times (0, 2, 4 , 6h) count mycelium formation rate, the data using SPSS 13.0 software for statistical analysis. Results Three kinds of oxidants could inhibit the formation of CAL mycelium. H 2 O 2, diamide and menaquinone inhibited mycelial growth in a dose-dependent manner. When the concentrations of H2O2, diamide and menaquinone were 30 mmol / L, CAL mycelium The rate of mycelial growth began to decline, the mycelium formation rate was 67.0%, 58.0%, 49.0% respectively. When the H2O2, diamide and menadione treatment 2h, CAL mycelium formation rate began to decline, the formation rates were 73.0%, 59.0% , 51.0%. CONCLUSION The three oxidants inhibited the formation of CAL mycelium in a concentration-dependent and time-dependent manner. Menaquinone inhibited the formation of CAL mycelium most strongly and the mycelium formation rate of standard strain was lower than that of clinical isolates.