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cDNA fragment of fertility gene MS2 from cotton was cloned by RT-PCR approach, it was highly homologous with relevant genes of Brassica napus and Arabidopsis thaliana. According to the principles of constructing RNAi vector, sense and antisense fragments of MS2 gene carrying restriction endonuclease recognition sites were amplified via PCR technique, ligated with the first intron of upland cotton chinase gene, then inserted into artificially modified plant expression vector pBI121, yielding RNAi vector pBGP12MSIn. The results showed that RNAi vector pBGP12MSIn harboring MS2 gene driven by anther specific promoter BGP was successfully constructed. Our results laid a foundation for studying the function of this gene and genetic transformation of plant male sterile lines.
cDNA fragment of fertility gene MS2 from cotton was cloned by RT-PCR approach, it was highly homologous with relevant genes of Brassica napus and Arabidopsis thaliana. According to the principles of constructing RNAi vector, sense and antisense fragments of MS2 gene carrying restriction endonuclease recognition sites were amplified via PCR technique, ligated with the first intron of upland cotton chinase gene, then inserted into artificially modified plant expression vector pBI121, yielding RNAi vector pBGP12MSIn. The results showed that RNAi vector pBGP12MSIn harbor MS2 gene driven by anther specific promoter BGP was successfully constructed. Our results laid a foundation for studying the function of this gene and genetic transformation of plant male sterile lines.