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目的探讨不同染尘剂量大鼠矽肺模型血清硅元素水平的变化情况,并与反映肺内早期炎症变化的细胞因子血清克拉拉细胞蛋白(Clara cell protein,CC16)进行比较,以弄清它们在矽肺发病中的意义和价值。方法雄性Wistar大鼠分为28组,按不同染尘剂量(10 mg/ml、30 mg/ml、50 mg/ml、70 mg/ml)染尘,而后在不同时间点(染尘后1 d、3 d、5 d、7 d、14 d、21 d和28 d)处死动物,采取血液及肺组织,用电感耦合等离子体质谱仪(ICP-MS)检测血清中硅元素含量,酶联免疫吸附测定法(ELISA法)测定大鼠血清中CC16含量。结果随着染尘剂量增加和染尘时间延长,大鼠血清硅元素的水平呈现逐渐增高的趋势;CC16含量于染尘后即开始降低,第7天开始与对照组相比差异有统计学意义(P<0.05)。并随着染尘时间延长和剂量增加,其含量亦不断减少,但血清硅元素的变化不论是从时间上还是从剂量上比较,均早于CC16。结论矽肺模型大鼠血清硅元素水平在早期低剂量染尘时即明显升高,且早于CC16的变化,二者的变化具有剂量和时间依赖性,提示血清硅元素、CC16水平变化可能为矽肺的早期诊断提供生物学依据,具有一定的临床价值。
Objective To investigate the changes of serum silicon levels in silicotic models of different doses of dust and to compare with the serum levels of Clara cell protein (CC16), which reflect the early inflammatory changes in the lungs, The significance and value of the disease. Methods Male Wistar rats were divided into 28 groups and were exposed to different doses of dust (10 mg / ml, 30 mg / ml, 50 mg / ml, 70 mg / ml). At different time points , 3 d, 5 d, 7 d, 14 d, 21 d and 28 d), blood and lung tissue were taken and the contents of silicon in serum were determined by inductively coupled plasma mass spectrometry (ICP-MS) Immunoadsorption assay (ELISA method) to determine the content of CC16 in rat serum. Results With the increase of dust dose and prolonged exposure time, the level of serum silicon in serum increased gradually. The content of CC16 began to decrease after the exposure to dust, and the difference was statistically significant from the 7th day to the control group (P <0.05). With the prolongation of dust exposure and dose increase, the content of serum silicon decreased continuously. However, the change of serum silicon was earlier than that of CC16, both in time and dose. Conclusion The level of serum silicon in silicosis model rats significantly increased at early low dose of dust exposure, and earlier than the change of CC16, the changes of both were dose-dependent and time-dependent, suggesting that the changes of serum silicon and CC16 may be caused by silicosis The early diagnosis provides a biological basis, has a certain clinical value.