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目的：探讨ｒＩＬ－２体外激活骨髓细胞产生细胞毒活性的作用机制．方法：取人新鲜骨髓中单个核细胞，体外与ｒＩＬ－２孵育２４ｈ及４８ｈ，即激活骨髓（ＡＢＭ），同时设不加ｒＩＬ－２组（ＮＢＭ）为对照．分别检测ＡＢＭ，ＮＢＭ的细胞毒活性、膜表面标志（ｍＴＮＦ－α，ＣＤ１１ｂ）及培养液中ＴＮＦ－α水平，并进行比较观察和相关性分析．结果：ＡＢＭ对ＨＬ－６０细胞的细胞毒活性较ＮＢＭ明显增强，ｍＴＮＦ－α，ＣＤ１１ｂ的表达能力及培养液中ＴＮＦ－α水平明显增高（Ｐ＜０．０５），且ＡＢＭ的ＣＤ１１ｂ表达水平与培养上清中ＴＮＦ－α水平，ＴＮＦ－α水平与同时相的细胞毒活性之间均呈密切正相关（Ｐ＜０．０１）．结论：ｒＩＬ－２激活骨髓细胞分泌释放ＴＮＦ－α参与了ＡＢＭ的杀肿瘤活性，可能与骨髓单核巨噬细胞、中性粒细胞和ＮＫ细胞被激活有关．
Objective: To investigate the mechanism of cytotoxic activity of rIL-2 activated bone marrow cells in vitro. Methods: Mononuclear cells from fresh bone marrow were harvested and incubated with rIL-2 in vitro for 24 h and 48 h, respectively, to activate bone marrow (ABM). Meanwhile, no rIL-2 group (NBM) was used as control. The cytotoxic activity of ABM and NBM, the membrane surface marker (mTNF-α, CD11b) and the level of TNF-α in the culture medium were detected respectively. The comparative observation and correlation analysis were performed. Results: The cytotoxicity of ABM to HL-60 cells was significantly higher than that of NBM, the expression of mTNF-α and CD11b was significantly increased (P <0.05), and the level of CD11b The level of TNF-α and the level of TNF-α in the culture supernatant were positively correlated with the cytotoxicity of the same phase (P <0.01). CONCLUSION: TNF-α secreted by rIL-2-activated myeloid cells is involved in the tumorigenicity of ABM. It may be related to the activation of mononuclear macrophages, neutrophils and NK cells.