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自然室温条件下用柱子淋洗模拟研究了接氧化亚铁硫杆菌(Thiobacillusferrooxidans,简称TF菌)时磷酸铁膜抑制黄铁矿氧化的效果。试验结果首次指出:用KH2PO4与H2O2淋洗黄铁矿,在其表面形成的磷酸铁膜不能抑制黄铁矿的生物氧化,反而在一定程度上加速其氧化进程。经包膜后的黄铁矿矿石柱子间歇循环淋洗一年后的收集液中累积量高达337561mg;为对照组的16倍,是杀菌剂处理的54倍。收集液的pH也从初始的740下降至230,外观呈深棕红色。收集液中其他元素的含量与对照组的相近,但均远高出杀菌剂处理的。一年后矿石样品经扫描电子显微镜观察,对照组和磷酸盐包膜的矿石样品表面布满细小洞穴;而杀菌剂处理的仍呈光洁状,说明TF菌对黄铁矿样品有强烈的侵蚀作用。可见,用磷酸铁膜法抑制黄铁矿氧化在野外矿区微生物作用不可避免的情况下难以取得预期效果。
Natural column temperature was used to simulate the leaching of bacteria when connected with Thiobacillus ferrooxidans (Thiobacillusferrooxidans, referred to as T F bacteria) inhibition of pyrophosphate iron oxidation effect. The experimental results indicated for the first time that the iron phosphate film formed on the surface of pyrite by KH2PO4 and H2O2 was not able to inhibit the biooxidation of pyrite, but accelerated the oxidation process to a certain extent. After the enveloping pyrite ore column intermittent leaching one year after the accumulation of liquid accumulation of up to 33,756 1mg; 1 6 times the control group, 54 times the fungicide treatment. The pH value of the collected liquid also decreased from 740 to 2.30 and the appearance of dark reddish brown. The contents of other elements in the collection solution were similar to those in the control group, but were much higher than that of the fungicide. One year later, the ore samples were observed by scanning electron microscopy. The surface of control samples and phosphate-coated ore samples were covered with small caves. However, the fungicide treatment was still smooth and clean, indicating that TF bacteria had a strong Erosion effect. It can be seen that the inhibition of pyrite oxidation by the iron phosphate film method is difficult to obtain the desired effect in the inevitable case of microorganisms in the field mining area.