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本研究通过实验用15个双列杂交组合和11个单因子杂交组合及其亲本自交系来测定根腐病抗性的遗传。试验在德国南部六个不同环境(指地点和年份的组合)中进行,其中四个是用P.herpotrichoides,F.culmorum或M.nivale进行人工接种,另外两个则为田间自然发病。在乳熟期,采用9个等级对每个实验小区的50个茎秆发生根腐病的程度进行评价。结果表明,所有材料的遗传方差均达到显著水平,遗传力估计值也很高(h2=0.82—0.92),一般配合力(GCA)是杂种间遗传性变异的主要来源,而特殊配合力则可忽略。同时还发现环境作用不容忽视,遗传—环境的交互作用很强,品系本身的特性和GCA效应之间的表型相关也很强(r=0.9),而杂种优势则不重要。为了优化病害评估,对变化的茎秆数、环境数和h2做了重复估计。在两个环境中对自交系进行试验,在四个环境中对单系进行试验,试验均设二次重复,每个实验小区15株的样本容量得到的h2约为0.7。最后,根据品质本身的特性,对非特定的根腐病病变进行了鉴定,结果表明,在杂种黑麦的选育中,针对根腐病的抗性进行选育是可以获得显著进展的。
In this study, the inheritance of root rot resistance was determined by using 15 pairs of crosses and 11 single-factor crosses and their parents inbred lines. The experiment was conducted in six different environments in southern Germany, referring to the combination of place and year, of which four were P. herpotrichoides, F. culmorum or M. nivale artificial inoculation, while the other two natural incidence of the field. At milking stage, nine grades were used to assess the extent of root rot in 50 stems in each experimental plot. The results showed that the genetic variance of all the materials reached a significant level, and the heritability estimates were also high (h2 = 0.82-0.92). General combining ability (GCA) was the main source of genetic variation among hybrids, while the special Combine force can be ignored. At the same time, it was also found that the role of environment should not be neglected. The genetic-environment interaction was very strong. The phenotypic correlation between the characteristics of the lines and the GCA effect was also strong (r = 0.9), while the heterosis was not important. In order to optimize the disease assessment, repeated estimations were made of the number of stems changed, the number of environments and h2. The inbred lines were tested in two environments, and the single lines were tested in four environments. The experiments were repeated twice. The h2 obtained from the sample size of 15 strains in each experimental plot was about 0.7. Finally, according to the characteristics of the quality of the non-specific root rot disease were identified, the results show that in the breeding of hybrid rye, the resistance to root rot breeding can make significant progress.