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Soybean mosaic virus (SMV) is a severe disease in worldwide soybean production. A cross was made between Kefeng No.1 with broad spectrum resistance to SMV and Nannong 1138-2, a susceptible cultivar. The inheritance of resistance to SMV strain Sa prevailing in southern China was analyzed. Results of x~2 test from inoculation experiment on parents F1, F2 and F3 lines showed that the resistance to strain Sa was controlled by a single dominant gene Rsa. BSA method was adopted and 900 random 10-mer primers were used to amplify total DNA from resistant pool and susceptible pool in order to obtain polymorphic bands in two bulks.16 primers could generate polymorphic bands, of which OPW-05 and OPAS-06 could generate the most stable RAPD patterns. RAPD markers OPW-05_(660) and OPAS-06_(1800) were found to be linked to Rsa.Their order and genetic distance were OPAS-06(1800)22.2 cM Rsa 10.1 cM OPW-05_(660). Southern blotting showed that both OPAS-06_(1800) and OPW-05_(660) were low copy DNA in genomic DNA. OPW-05_(
A cross was made between Kefeng No.1 with broad spectrum resistance to SMV and Nannong 1138-2, a susceptible cultivar. The inheritance of resistance to SMV strain Sa prevailing in Southern China was analyzed. Results of x ~ 2 test from inoculation experiment on parents F1, F2 and F3 lines showed that the resistance to strain Sa was controlled by a single dominant gene Rsa. BSA method was adopted and 900 random 10-mer primers were used to amplify total DNA from resistant pool and susceptible pool in order to obtain polymorphic bands in two bulks. 16 markers could generate polymorphic bands, of which OPW-05 and OPAS-06 could generate the most stable RAPD patterns. RAPD markers OPW-05_ (660) and OPAS-06_ (1800) were found to be linked to Rsa.Their order and genetic distance were OPAS-06 (1800) 22.2 cM Rsa 10.1 cM OPW- 05_ (660). Southern blotting showed that both OPAS-06_ (1800) and OPW-05_ (660) were low copy DNA in gen omic DNA. OPW-05_ (