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[目的]为职业性膀胱癌高危人群的监护及膀胱癌的临床诊断探索一种特异、可靠的手段。[方法]应用核酸探针杂交技术,免疫组化法及定量PCR方法对50 例膀胱癌高危人群,18 例膀胱癌患者及30 例健康人群进行外周血白细胞中AN43 基因及其m RNA 的定量PCR检测,并与尿细胞病理学检查结果进行比较。[结果] 职业性膀胱癌高危人群AN43 基因的检出率及其m RNA的定量PCR读数均明显高于对照人群而低于膀胱癌患者。[结论] AN43基因检测及其m RNA 的定量PCR法不仅可作为职业性膀胱癌高危人群癌前病变控制的监护指标,也可作为膀胱癌早期诊断指标之一
[Objective] To explore a specific and reliable method for the monitoring of high-risk population of occupational bladder cancer and the clinical diagnosis of bladder cancer. [Method] The nucleic acid probe hybridization, immunohistochemistry and quantitative PCR were used to detect the expression of AN43 gene and its m RNA in 50 cases of high-risk of bladder cancer, 18 cases of bladder cancer and 30 healthy people Test, and compared with the urine cytopathology test results. [Results] The detection rate of AN43 gene and the quantitative PCR reading of m RNA in the high risk population of occupational bladder cancer were significantly higher than those in the control group and lower than those in the bladder cancer patients. [Conclusion] The detection of AN43 gene and its m RNA quantitative PCR method can not only be used as a monitoring index for the control of precancerous lesions in high-risk population of occupational bladder cancer, but also as one of the indicators of early diagnosis of bladder cancer