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我们于1959—1961年利用示踪原子法进行了柑桔磷素营养生理问题的研究。以酸桔砧、雪柑穗嫁接后一年生的橙苗(Citrus sinensis Osbeck)为盆栽试验的材料。田问试验则是以柠檬砧、暗柳穗定植后两年生的橙树(C. sinensis) 为材料。使用P~(32)标记过磷酸钙的热水浸提液。每处理2株,重复1—2次,共4—6株。测定示踪磷的强度是用带有稳压装置的定标器,每次取三个平行样品,测定重复2次。初步结果如下: 一、橙苗定植后对基肥中示踪磷的吸收 试验分三个处理:Ⅰ,每盆施石灰200克加入示踪磷。Ⅱ.不施石灰,单施等量的示踪磷。Ⅲ.不施石灰,但施入5斤猪粪与垃圾的混合肥,并加入等量的示踪磷。两星期后测定每盆土壤的pH值。试验结果如下图所示(图中示踪磷强度系在树上直接测定的结果)。
In 1959-1961 we used the tracer atomic method to study the physiological and nutritional problems of phosphorus in citrus. Citrus sinensis Osbeck, an annual rootstock of Citrus sinensis, was grafted with aphids and Citrus grandiflora. Field test is based on lemon anvil, dark willow spike two years after planting orange tree (C. sinensis) as material. P ~ (32) labeled hot water extract of superphosphate. Each treatment 2, repeated 1-2 times, a total of 4-6 strains. Determination of tracer phosphorus intensity is a regulator with a voltage regulator, each taking three parallel samples were measured twice. Preliminary results are as follows: First, orange plantation after basal fertilizer on the tracer phosphorus absorption test divided into three treatments: Ⅰ, 200 grams per pot of lime added to trace the phosphorus. Ⅱ. Without lime, apply the same amount of tracer phosphorus. III. Without lime, apply 5 kg of mixed fertilizer of pig manure and rubbish and add the same amount of tracer phosphorus. Two weeks later, the pH of each pot of soil was measured. The results of the experiment are shown in the following figure (the trace of phosphorus in the figure is the result of the direct determination on the tree).