论文部分内容阅读
目的研究实验性自身免疫性泪腺炎（ＥＡＤ）的发病机理为实验性治疗奠定基础。方法利用ＤＥＡＥ５２纤维素柱层析提纯牛泪腺抗原，将活性抗原配以完全福氏佐剂免疫Ｗｉｓｔａｒ大鼠。结果提纯的活性牛泪腺抗原分子量为４３ｋＤ或６７ｋＤ。免疫２０只Ｗｉｓｔａｒ大鼠，１６只发生ＥＡＤ（８０％）。病理检查证实，腺周慢性炎性细胞浸润，局灶形成，小叶内腺泡破坏，单核细胞浸润。结论经ＤＥＡＥ５２纤维素柱层析法提纯牛泪腺活性抗原可用于ＥＡＤ模型的建立，牛泪腺抗原与大鼠泪腺组织之间存在共同抗原可发生交叉反应。
Objective To study the pathogenesis of experimental autoimmune dacryoadenitis (EAD) to lay the foundation for experimental treatment. Methods DEAE52 cellulose column chromatography was used to purify cattle lacrimal gland antigen. Wistar rats were immunized with the active antigen and complete Freund’s adjuvant. Results Purified active cow lacrimal gland antigen molecular weight of 43kD or 67kD. Twenty Wistar rats were immunized, and EAD occurred in 16 (80%). Pathological examination confirmed that chronic glandular inflammatory cell infiltration, focal formation, lobular destruction of acinar, mononuclear cell infiltration. Conclusion DEAE52 cellulose column chromatography purification of lacrimal gland active antigen can be used for the establishment of EAD model, cattle lacrimal gland antigen and rat lacrimal gland tissue common antigen cross-reaction occurs.