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目的 探讨表皮生长因子 (EGF)及其受体(EGFR)、原癌基因c fos和c myc在不同胎龄的胎儿和少儿皮肤中的基因表达变化特征及其可能的生物学意义。方法 用病理学技术检测不同发育阶段皮肤的结构特征后 ,提取 18例不同胎龄(13～ 32周 )的胎儿皮肤和 6例少儿皮肤的总RNA ,用RT PCR方法检测这 4种基因在不同组织中的表达变化规律。结果 在早期妊娠胎儿的皮肤中 ,EGF、EGFR、c fos和c myc基因表达较弱 ,随着胎儿的生长和发育 ,皮肤内这 4种基因表达逐渐增强 ,在少儿皮肤中 ,EGF、EGFR、c fos和c myc基因表达进一步升高 ,基因表达量分别为晚期妊娠胎儿皮肤的 1 2、1 1、1 3和 1 6倍。结论 EGF与其受体结合后引起的信号通路可能对皮肤的发生、结构功能的维持以及伤后修复具有重要意义 ;EGF、EGFR、c fos和c myc基因在早期妊娠胎儿皮肤中低表达可能与创面无瘢痕修复密切相关。
Objective To investigate the gene expression changes of epidermal growth factor (EGF) and its receptor (EGFR), proto-oncogene c fos and c myc in fetal and juvenile skin of different gestational age and its possible biological significance. Methods The total RNA of 18 cases of fetus with different gestational age (13 ~ 32 weeks) and 6 cases of children’s skin was extracted by pathological technique. The total RNA of 18 cases Changes in the expression of the organization. Results The expression of EGF, EGFR, c fos and c myc in the fetal skin of early pregnancy was weak. With the growth and development of the fetus, the expressions of these four genes in the skin gradually increased. In the skin of children, the expression of EGF, EGFR, c fos and c myc gene expression was further increased, gene expression levels were late pregnancy fetal skin 12,1,1 and 1 6 times. Conclusions The signaling pathways induced by the binding of EGF to its receptors may play an important role in the development of the skin, the maintenance of structural function and the repair of wounds. The low expression of EGF, EGFR, c fos and c myc genes in fetal skin of early pregnancy may be related to the wound surface No scar repair is closely related.