论文部分内容阅读
观察体外细胞株ＰＧ、ＰＡａ细胞的ｕ－ＰＡ，ＰＡＩ－１活性和抗原表达，结果发现ＰＧ、ＰＡａ细胞均能够产生ＰＡ及ＰＡＩ，免疫细胞化学法定位ＰＡ主要为ｕ－ＰＡ，ＰＡＩ主要为ＰＡＩ－１。在ＰＧ为ｕ－ＰＡ高表达，而ＰＡａ为低ｕ－ＰＡ表达，ＰＡＩ－１在ＰＧ、ＰＡａ细胞中均高表达。对ＰＧ瘤细胞在ＢＡＬＢ／ＣＡ裸鼠皮下种植性瘤组织的免疫组化结果显示，ｕ－ＰＡ阳性～强阳性表达，ＰＡＩ－１为阴性表达。说明ｕ－ＰＡ是ＰＧ、ＰＡａ高低不同的转移能力的重要机制之一。
Observed the in vitro cell line PG, PAa cells u-PA, PAI-1 activity and antigen expression, found that PG, PAa cells can produce PA and PAI, immunocytochemistry localization of PA mainly u-PA, PAI is mainly PAI -1. In PG for u-PA high expression, and PAa low u-PA expression, PAI-1 in PG, PAa cells are highly expressed. The immunohistochemical results of PG tumor cells in subcutaneous implanted tumor tissues of BALB/CA nude mice showed that u-PA was positively-strongly positively expressed and PAI-1 was negatively expressed. It shows that u-PA is one of the important mechanisms for the different transfer ability of PG and PAa.