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目的:探讨苦参素对实验性肝纤维化大鼠的肝组织核转录因子-κB(NF-κB)和结缔组织生长因子(CTGF)表达的影响。方法:利用四氯化碳(CCl4)诱导大鼠肝纤维化模型。SD雄性大鼠45只,随机分为苦参素组(60 mg.kg-1 ip,1次/d,连续10周)、模型组和正常对照组(ip溶液橄榄油)于造模同时开始给予。10周后剖取肝脏采用HE染色观察各组大鼠肝组织病理改变;免疫组织化学法检测NF-κB,CTGF在肝组织内的表达。结果:与模型组大鼠比较,苦参素组大鼠肝纤维化水平明显减轻(P<0.01),肝组织NF-κB,CTGF表达明显降低(P<0.01)。结论:苦参素可抑制模型大鼠肝纤维化的形成和发展,其机制可能与下调肝内NF-κB,CTGF表达有关。
Objective: To investigate the effect of oxymatrine on the expression of nuclear factor-κB (NF-κB) and connective tissue growth factor (CTGF) in experimental hepatic fibrosis rats. Methods: Carbon tetrachloride (CCl4) was used to induce hepatic fibrosis in rats. Forty-five male Sprague Dawley rats were randomly divided into three groups: matrine group (60 mg.kg-1 ip, once a day for 10 weeks), model group and normal control group (ip solution olive oil) give. After 10 weeks, the liver was taken for HE staining to observe the pathological changes of liver tissue in each group. The expression of NF-κB and CTGF in liver tissues was detected by immunohistochemistry. Results: Compared with the model group, the levels of liver fibrosis were significantly reduced in the kushenin group (P <0.01), and the expressions of NF-κB and CTGF in the liver tissue were significantly decreased (P <0.01). Conclusion: Oxymatrine can inhibit the formation and development of liver fibrosis in rats. The mechanism may be related to the down-regulation of NF-κB and CTGF in the liver.