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本研究利用LC-MS/MS方法考察单次灌胃给予五酯片后其木脂素活性成分在大鼠肠道、肝脏的分布特点及随时间的变化规律。雄性SD大鼠灌胃给予五酯片0.25 g·kg-1后,于给药后0.25、1.5、4、6、10和24 h分批取大鼠肝脏及肠道组织,样本处理后进行LC-MS/MS分析。结果表明,五酯片各木脂素活性成分在大鼠肝脏、肠道的浓度随着五酯片给药时间的推移而降低;灌胃给予五酯片0.25 h后,五酯片各木脂素活性成分在大鼠肠道及肝脏中的浓度最高;灌胃给予五酯片24 h后,大鼠肠道及肝脏中检测不到五酯片各木脂素活性成分,提示五酯片各木脂素活性成分在脏器中消除较快,不容易蓄积。五味子酯甲、甲素、醇乙在肠道中的浓度高于其在肝脏中的浓度,提示灌胃给予五酯片可能对大鼠肠道、肝脏代谢酶作用强度存在差异。综上所述,单次灌胃给予五酯片,其木脂素活性成分不容易在大鼠肝脏及肠道蓄积,且大多数木脂素在肠道的浓度高于在肝脏的浓度。
In this study, the LC-MS / MS method was used to investigate the distribution of lignans active components in the intestine and liver of rats and the changes with time after administration of pentasodium salt. Male Sprague-Dawley rats were given intragastric administration of pentacetin 0.25 g · kg-1, and the liver and intestine of rats were divided into groups at 0.25, 1.5, 4, 6, 10 and 24 h after administration. LC -MS / MS analysis. The results showed that the concentration of each lignan active ingredient in the pentagastrin in the liver and intestine of the rats decreased with the administration time of the pentacene tablets. After the pentacene tablets were administered orally for 0.25 h, The active ingredient in the intestine and liver of rats had the highest concentration. After oral administration of pentacene tablets for 24 h, the activity of each of the five lignans in the intestine and liver of rats was not detected, Lignans active ingredients in organs to eliminate faster, not easy to accumulate. Schisandra ester A, A, B in the intestine in the concentration higher than its concentration in the liver, suggesting that intragastric administration of pentasil may have different effects on the intestine and liver metabolic enzymes in rats. In summary, a single oral administration of penta-ester tablets, the lignan active ingredient is not easy to accumulate in the liver and intestine, and most of the lignans in the gut concentration is higher than in the liver concentration.