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目的研究明日叶查尔酮(Ashitaba Chalcone,AC)对2型糖尿病大鼠胰腺细胞Bcl-2和Bax蛋白表达的影响。方法将用高脂饲料喂养加小剂量链脲佐菌素腹腔注射诱发的2型糖尿病雄性大鼠随机分为4个组,高、中、低剂量AC组高脂饲料喂养的基础上分别每日经口灌胃给予AC 30、10和5 mg/kg,糖尿病对照组只喂饲高脂饲料,正常对照组喂养普通饲料,实验期4周。用葡萄糖氧化酶法测定空腹血糖含量,放射免疫法测定血清胰岛素含量,免疫组化法检测胰腺细胞Bcl-2和Bax蛋白的表达水平,噻唑蓝(MTT)法检测胰腺细胞的增殖活性。结果糖尿病对照组Bcl-2和Bax表达水平分别为(0.407±0.032)和(0.615±0.050),高剂量AC组则为(0.488±0.020)和(0.544±0.026),差异均有统计学意义(P<0.05);糖尿病对照组胰腺细胞增殖活性为(0.244±0.023),高剂量AC组较其明显升高(P<0.05)。结论 AC可上调胰腺细胞Bcl-2蛋白的表达,下调Bax蛋白的表达,对2型糖尿病大鼠胰腺细胞损伤具有一定的拮抗作用。
Objective To investigate the effects of tomorrow on the expression of Bcl-2 and Bax in pancreatic cells of type 2 diabetic rats induced by Ashitaba Chalcone (AC). Methods Male rats with type 2 diabetes induced by intraperitoneal injection of high-fat diet plus small dose of streptozotocin (STZ) were randomly divided into 4 groups. On the basis of high, medium and low-dose high-fat diet, Oral administration of AC 30, 10 and 5 mg / kg, diabetic control group fed only high-fat diet, normal control group fed normal feed, the experimental period of 4 weeks. Fasting blood glucose levels were measured by glucose oxidase method. Serum insulin levels were measured by radioimmunoassay. Expressions of Bcl-2 and Bax proteins were detected by immunohistochemistry. Proliferation of pancreatic cells was assayed by MTT assay. Results The expressions of Bcl-2 and Bax in the diabetic control group were (0.407 ± 0.032) and (0.615 ± 0.050), respectively, and those in the high-dose AC group were (0.488 ± 0.020) and (0.544 ± 0.026) P <0.05). The proliferative activity of pancreatic cells in diabetic control group was (0.244 ± 0.023), higher than that in high-dose AC group (P <0.05). Conclusion AC can up-regulate the expression of Bcl-2 in pancreatic cells and down-regulate the expression of Bax protein, which can antagonize the pancreatic cell injury in type 2 diabetic rats.