论文部分内容阅读
[目的]研究钩状木霉Th12菌株最适的液体发酵条件,为研发和开发生物防治木霉制剂提供理论基础。[方法]采用稀释土壤平板法以及平板对峙法筛选、鉴定出钩状木霉Th12菌株,并考察了碳源、氮源、发酵时间、发酵温度、初始pH、接菌量、装瓶量、摇床转速对木霉菌丝产量的影响。[结果]木霉Th12菌株的最适液体发酵条件为以葡萄糖为碳源、以蛋白胨为氮源、发酵温度为25℃、初始pH为6、接菌量为0.8ml、装瓶量为50ml、摇床转速为180r/min的培养液中培养3d,菌丝产量最高。[结论]木霉Th12菌株在最佳液体培养条件中能最大限度地提供用于草坪镰孢枯萎病和褐斑病病害防治的菌丝体量。
[Objective] The study aimed to study the optimal liquid fermentation conditions of Trichoderma viride Th12 and provide the theoretical basis for the research and development of biological control of Trichoderma preparations. [Method] The Thii genus Th12 strain was identified by using the diluted soil slab method and the slab confrontation method. The effects of carbon source, nitrogen source, fermentation time, fermentation temperature, initial pH, Effect of bed speed on the yield of Trichoderma mycelia. [Result] The optimum liquid fermentation conditions for Trichoderma Th12 strain were glucose as carbon source, peptone as nitrogen source, fermentation temperature of 25 ℃, initial pH of 6, volume of inoculation of 0.8 ml, bottling volume of 50 ml, Shaker bed speed of 180r / min culture medium cultured 3d, mycelium yield the highest. [Conclusion] The Trichoderma Th12 strain can provide the maximum amount of mycelium for controlling Fusarium oxysporum f. Sp. And brown spot disease in the best liquid culture conditions.